Reagent for labelling nucleic acids

Details Reagent for labelling nucleic acids Reagent for labelling nucleic acids

Filed on

2005-04-05

Date issued

2005-04-05

Examiner

Witz, Jean C. (Department: 1651)

Patent Class

Organic compounds -- part of the class 532-570 series

SubClass

Organic compounds

SubSubClass

Carbohydrates or derivatives

Other Related Categories

C435S006120

Type

Reexamination Certificate

Status

active

Patent number

06875850

Description

ABSTRACT:
The present invention concerns a labelling reagent in which the label is bound via an amide bond and a linker to a residue of the molecule which is essentially characterized in that the N atom of the amide bond and the label are linked together directly by a covalent bond. In particular these are phosphoramidites or reactive supports suitable for nucleic acid synthesis. The invention also concerns processes for the production of such supports from suitable precursors.

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patent: WO9746707 (1997-12-01), None
Asseline et al. “Solid Phase Preparation of 5′,3′-heterobifunctional Oligodeoxynucleotides Using Modifed Solid Supports” Tetrahedron (1992) 48(7): 1233-54.*
Dubey, Igor et al., “Modification of the Thiourea Linkage of a Fluorescein-Oligonucleotide Conjugate to a Guanidinium Motif during Ammonia Deprotection”, Bioconjugate Chem. 1998, vol. 9, pp 627-632 (6) pp.
Igor Dubey, et al., “Modification of the Thiourea Linkage of a Fluorescein-Oligonucleotide Conjugate to a Guanidinium Motif during Ammonia Deprotection”, Bioconjugate Chem. 1998, 9, 627-632.
P.K. Ghosh, et al., “Advances in tionalization of Polymer Supports for Synthesis and Modification of oligonucleotides” J. Indian Chem. Soc., vol. 75, Apr. 1998, pp. 206-218.
Ahmad Hansan et al., “Photolabile Protecting Groups for Nucleosides: Synthesis and Photodeprotection Rates” Tetrahedron, vol. 53, No. 12, pp. 4247-4264, 1997.
H. Schaller, et al.. “Studies on Polynucleotides. XXIV. The Stepwise Synthesis of Specific Deoxyribopolynucleotides (4). Protected Derivatives of Deoxyribonucleosides and New Syntheses of Deoxyribonucleoside-3 Phosphates”, Syntheses of Deoxyribonucleside-3 Phosphates, Dec. 5, 1963, pp. 3821-3827.
M.S. Shchepinov, et al., “Steric Factors Influencing Hybridisation of Nucleic Acides to Oligonucleotide Arrays” 1997 Oxford University Press, Nucleic Acids Research, 1997, vol. 25, No. 6 (pp. 1155-1161).
Christian Wojczewski, et al., “Fluorescent Oligonucleotides- Versatile Tools as Probes and Primers for DNA and RNA Analysis” Synlett 1999, No. 10, 1667-1678.

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