Detection and differentiation of specific strains of citrus...

Details Detection and differentiation of specific strains of citrus... Detection and differentiation of specific strains of citrus...

Filed on

2005-03-22

Date issued

2005-03-22

Examiner

Sitton, Jehanne (Department: 1634)

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Measuring or testing process involving enzymes or...

SubSubClass

Involving nucleic acid

Other Related Categories

C435S091100, C435S091200, C536S023100, C536S024300

Type

Reexamination Certificate

Status

active

Patent number

06869761

Description

ABSTRACT:
Nucleic acid probes for detecting different strains of Citrus Tristeza Virus (CTV) were made and shown to be highly sensitive, specific, and selective. The invention also concerns a method of detection, a method of identifying novel strains of CTV, and a detection kit which employs the subject probes.

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patent: 6017699 (2000-01-01), Jordan
patent: 6037124 (2000-03-01), Matson
patent: 6140046 (2000-10-01), Niblett
H Pappu et al., Virus Genes,“Comparative Sequence Analysis of the Coat Proteins of Biologically Distinct Citrus Tristeza Closterovirus Isolates,” 1993, 7:3, pp. 255-264.*
M Mawassi et al., Virus Genes, “Nucleotide Sequence of the Coat Protein Gene of Citrus Tristeza Virus:Comparison of Biologically Diverse Isolates Collected in Israel,” 7:3, 265-275.*
B Cevik et al., “Detection and differentiation of strains of citrus tristeza closterovirus using a point mutation and minor sequence differences in their coat protein genes,” 1996, vol. 86, No. 11, Abstract Only.*
Nolasco et al., “Sensitive CTV diagnosis using immunocapture, reverse transcriptional polymerase chain reaction and an exonuclease flourescent probe assay,” Fruits, 52: 391-396, 1997.
Rowhani, et al., “Development of a Sensitive Colorimetric-PCR Assay for Detection of Viruses in Woody Plants,” Plant Disease, 82: 880-884, 1998.
Schmid M. and G. Schalasta, “A Rapid and Reliable PCR Based Method for Detecting the Blood Coagulation Factor V Leiden Mutation,” Biochemica, 3: 12-15, 1997.
Roche Molecular Biochemicals, 2000 Biochemicals Catalog, “PCR ELISA”.

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