Expression system for cloning toxic genes

Details Expression system for cloning toxic genes Expression system for cloning toxic genes

2005-04-19

2005-04-19

Leffers, Gerry (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S252100, C435S252300, C435S320100, C435S325000, C435S348000, C435S455000, C435S069100, C536S023100, C536S024100, C536S024200

Reexamination Certificate

active

06881558

ABSTRACT:
Methods of cloning and/or amplifying toxic genes in bacteria using a vector which amplifies the toxic gene in bacteria and also allows subsequent expression in mammalian systems is provided. A vector having an origin of replication, a first promoter, a polylinker, a second promoter in reverse orientation with respect to the first promoter, a poly adenylation signal, and a gene encoding a selectable marker, and optionally an enhancer operably connected to the first promoter, and/or a nucleotide sequence encoding a toxic protein is also provided.

REFERENCES:
patent: 5385839 (1995-01-01), Stinski
pBK-CMV information from Stratagene catalog.*
B Matthey et al., Gene, “A new series of pET-derived vectors for high efficiency expression of Pseudomonas exotoxin-bases fusion proteins,” 1999, 229, pp. 145-153.*
MI Bukrinsky et al., Gene, “Multicopy expression vector based on temperature-regulated lac repressor:expression of human immunodeficiency virus env gene inEscherichia coli,” 1988, 70, pp. 415-417.*
Expression Systems and Vectors, Invitrogen Catalog 1994, 5:41 & 66.*
Ratagene, Cloning Systems 1994, Creating the tools for the creative mind,pp. 18, 19 &45.*
Novagen, pET-27b(+) Vector, 1998, READSEQ Sequence Conversion Results, pp. 1-4.

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