Novel reagent and method for the determination of urea in biolog

Textiles: fiber preparation

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G01N 3114

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active

041013824

ABSTRACT:
The ease and speed of known enzymatic assays of micro-amounts of urea is improved by a novel reagent assay comprising: urease, buffers, and an indicator dye, the improvement wherein a mixed buffer system is present which mitigates against the effects of temperature changes during the assay, and a novel method of determining released ammonia with an indicator dye and spectrophotometer.

REFERENCES:
patent: 3119751 (1964-01-01), Chaney
patent: 3527674 (1970-09-01), Deutsch
patent: 3542649 (1970-11-01), Searcy
patent: 3635681 (1972-01-01), Rogers

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