Method of making an aldehyde dehydrogenase with gluconobacter

Details Method of making an aldehyde dehydrogenase with gluconobacter Method of making an aldehyde dehydrogenase with gluconobacter

2005-01-25

2005-01-25

Marx, Irene (Department: 1651)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S136000, C435S252100, C435S191000, C435S071200

Reexamination Certificate

active

06846660

ABSTRACT:
A new aldehyde dehydrogenase having the physico-chemical properties: molecular weight:150,000±6,000 or 230,000±9,000; substrate specificity active on aldehyde compounds; cofactors:pyrroloquinoline quinone and heme c; optimum pH: 7.0-8.5; and inhibitors: Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and EDTA, is derived from a microorganism belonging to the genusGluconobacter. Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genusGluconobacterwhich is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism. 2-Keto-L-gulonic acid (2-KGA) can be produced from L-sorbosone by contacting L-sorbosone with (i) the aldehyde dehydrogenase in the presence of an electron acceptor, (ii) aGluconobactermicroorganism capable of producing the aldehyde dehydrogenase in an aqueous medium under aerobic conditions or (iii) a cell-free extract of said microorganism, and in each case isolating the resulting 2-KGA from the reaction mixture.

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