Process for producing transaminase from E. coli ATCC 33849

Details Process for producing transaminase from E. coli ATCC 33849 Process for producing transaminase from E. coli ATCC 33849

1989-06-01

1992-07-14

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435849, 4352528, C12N 910, C12N 100, C12N 120

Patent

active

051302462

ABSTRACT:
A process for the preparation of a purified transaminase having a molecular weight of 20,000 to 250,000 daltons, an isoelectric point at a pH between 3.0 and 8.0, a pH optimum in a range from 5.0 to 10.0 and a substrate specificity for the transamination of (3-carboxy-3-oxo-propyl)-methyl-phosphinic acid or the esters thereof.
The production of the enzyme comprises cultivating E. coli ATCC 33849, disrupting the cultivated E. coli ATCC 33849, obtaining a supernatant therefrom containing the enzyme and isolating the transaminase by heating the supernatant at a temperature and for a time sufficient to denature proteins other than the transaminase and than finally removing the denatured proteins from the supernatant.

REFERENCES:
patent: 4525454 (1985-06-01), Rozzell
patent: 4826766 (1989-05-01), Rozzell
patent: 4859591 (1989-08-01), Walter et al.
Hanahan, J. Mol. Biol., 166:557-580 (1983).
The American Type Culture Collection Catalogue of Bacteria and Bacteria Pages, 17th Edition, p. 92 (1989).

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