Process for producing human epidermal growth factor and analogs

Details Process for producing human epidermal growth factor and analogs Process for producing human epidermal growth factor and analogs

1988-01-04

1991-04-02

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 435 91, 435701, 435 711, 435170, 4351721, 4351723, 435212, 4352523, 43525233, 4353201, 435844, 536 27, 935 10, 935 22, 935 23, 935 24, 935 47, 935 48, 935 49, 935 51, 935 59, 935 60, 935 72, 935 73, C12N 121, C12N 952, C12N 1512, C12N 1563, C12N 1570, C12P 2106

Patent

active

050046861

ABSTRACT:
Disclosed is a process for producing a fusion protein of epidermal growth factor (EGF) attached through a Glu-residue, treatment of the fusion protein with a Staphylococcus aureus protease specific for cleaving peptides at a Glu-linkage thereby producing large amounts of the 53 amino acid EGF sequence.
The treatment conditions optimize production of large amounts of the 53 amino acid sequence relative to the 51 amino acid sequence minus the 52 Leu and 53 Arg residues and take full advantage of the selectivity of the protease.

REFERENCES:
patent: 3917824 (1975-11-01), Camble et al.
patent: 4338597 (1982-07-01), Gilbert et al.
Brake et al., 1984, PNAS 81: 4642-4646.
Hogg, 1981, J. Biol. Chem., 256 (4): 1935-1939.

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