Method for the measurement of reverse transcriptase using immobi

Details Method for the measurement of reverse transcriptase using immobi Method for the measurement of reverse transcriptase using immobi

1994-03-08

1996-04-16

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

055081665

ABSTRACT:
A method is provided for the determination of the presence of a reverse transcriptase in a sample liquid. In an aqueous medium, at least an adenine ribopolynucleotide RNA template (poly A), an immobilized oligodeoxythyminenucleotide and a biotinylated deoxyuridine triphosphate are reacted in the presence of the sample liquid. After separation of the reacted and the unreacted biotinylated deoxyuridine triphosphate from each other, the reacted or the unreacted biotinylated deoxyuridine triphosphate is detected. Also provided is a method for the determination of infection of a patient to a retrovirus. The reaction is conducted in the presence of a sample body fluid and the reverse transcriptase. After the detection of the reacted or the unreacted biotinylated deoxyuridine triphosphate, the reverse-transcriptase-neutralizing and/or inhibiting antibody titer in the sample body fluid is determined.

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