Method for production of proteins in yeast

Details Method for production of proteins in yeast Method for production of proteins in yeast

1997-10-10

1999-08-17

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 435 711, 4352551, 4353201, 536 231, 536 234, C07H 2104, C12N 1563, C12N 1500, C12P 2100

Patent

active

059392878

ABSTRACT:
The present invention concerns the production of useful proteins on the growth medium of yeast by using the hsp150 protein or fragments or derivatives thereof as a carrier. The authentic hsp150 protein, encoded by the hsp150 gene of Saccharomyces cerevisiae, is secreted efficiently to the growth medium. In the new recombinant DNA vectors the gene encoding the desired protein is joined to the hsp150 nucleotide sequence. The fusion gene is transformed to a yeast host to express the respective fusion protein. The signal sequence of the hsp150 protein, or another sequence, guides the fusion protein to the secretory pathway, and the hsp150-carrier takes it to the medium, from where it is harvested. During the secretion protein, the protein product may or may not be detached from the hsp150 carrier by proteolytic enzymes, according to the occurrence or created cleavage sites at carrier-product junction.

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