Production of enzymes having desired activities by mutagenesis

Details Production of enzymes having desired activities by mutagenesis Production of enzymes having desired activities by mutagenesis

1996-05-22

1999-08-17

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435183, 435 691, 536 231, 536 232, C12Q 100, C12N 900

Patent

active

059392509

ABSTRACT:
Disclosed is a process for obtaining an enzyme having a specified enzyme activity derived from a heterogeneous DNA population by screening, for the specified enzyme activity, a library of clones containing DNA from the heterogeneous DNA population which have been exposed to directed mutagenesis towards production of the specified enzyme activity. Also disclosed is a process for obtaining an enzyme having a specified enzyme activity by screening, for the specified enzyme activity, a library of clones containing DNA from a pool of DNA populations which have been exposed to directed mutagenesis in an attempt to produce in the library of clones DNA encoding an enzyme having one or more desired characteristics which can be the same or different from the specified enzyme activity.

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Osuna et al. "Combinatorial mutagensis of three major groove-contacting residues of EcoRI: . . . " Gene 106, 7-12, 1991.
Dube et al. Artificial mutant generated by the insertion of random oligonucleotides into the putative . . . Biochemistry 30, 11760-11767, 1991.

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