Fumonisin detoxification compositions and methods

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S197000, C536S023200

Reexamination Certificate

active

06573075

ABSTRACT:

TECHNICAL FIELD
The present invention relates generally to the detection and isolation of fumonisin resistant organisms and to compositions and methods for the in vivo detoxification or degradation of fumonisin. This method has broad application in agricultural biotechnology and crop agriculture and in the improvement of food grain quality.
BACKGROUND OF THE INVENTION
Fungal diseases are common problems in crop agriculture. Many strides have been made against plant diseases as exemplified by the use of hybrid plants, pesticides and improved agricultural practices. However, as any grower or home gardener can attest, the problems of fungal plant disease continue to cause difficulties in plant cultivation. Thus, there is a continuing need for new methods and materials for solving the problems caused by fungal diseases of plants. These problems can be met through a variety of approaches. For example, the infectious organisms can be controlled through the use of agents that are selectively biocidal for the pathogens. Another method is interference with the mechanism by which the pathogen invades the host crop plant. Yet another method, in the case of pathogens that cause crop losses, is interference with the mechanism by which the pathogen causes injury to the host crop plant. Still another method, in the case of pathogens that produce toxins that are undesirable to mammals or other animals that feed on the crop plants, is interference with toxin production, storage, or activity. This invention falls into the latter two categories.
Since their discovery and structural elucidation in 1988 (Bezuidenhout S, Gelderblom W, Gorst-Allman C, Horak R, Marasas W, Spiteller B, Vleggaar R (1988) “Structure elucidation of the fumonisins, mycotoxins from
Fusarium moniliforme.” Journal Chem Soc, Chem Commun
1988: 743-745), fumonisins have been recognized as a potentially serious problem in maize-fed livestock. They are linked to several animal toxicoses including leukoencephalomalacia (Marasas W F O, Kellerman T S, Gelderblom W C A, Coetzer J A W, Thiel P (1988) “Leukoencephalomalacia in a horse induced by fumonisin B-1 isolated from
Fusarium moniliforme.” Onderstepoort Journal of Veterinary Research
55: 197-204; Wilson T M, Ledet A E, Owens D L, Rice L G, Nelson H A (1990) “Experimental liver disease in ponies associated with the ingestion of a corn-based ration naturally contaminated with fumonisin B
1
,” American Association of Veterinary Laboratory Diagnosticians: Abstracts
33
rd Annual Meeting
, Denver, Colo., Oct. 7-9, 1990., Madison, Wis., USA) and porcine pulmonary edema (Colvin B M, Harrison L R (1992) “Fumonisin-Induced Pulmonary Edema and Hydrothorax in Swine.”
Mycopathologia
117: 79-82). Fumonisins are also suspected carcinogens. (Geary W (1971)
Coord Chem Rev
7: 81; Gelderblom W C A, Kriek N P J, Marasas W F O, Thiel P G (1991) “Toxicity and Carcinogenicity of the Fusarium-Moniliforme Metabolite, Fumonisin-B1, in Rats.”
Carcinogenesis
12: 1247-1251; Gelderblom W C A, Semple E, Marasas W F O, Farber E (1992) “The Cancer-Initiating Potential of the Fumonisin-B Mycotoxins.”
Carcinogenesis
13: 433-437). Fusarium isolates in section Liseola produce fumonisins in culture at levels from 2 to >4000 ppm (Leslie J, Plattner R, Desjardins A, Klittich C (1992) “Fumonisin B1 production by strains from different mating populations of
Gibberella fujikoroi
(Fusarium section Liseola).”
Phytopathology
82: 341-345). Isolates from maize (predominantly mating population A ) are among the highest producers of fumonisin. (Leslie et al., supra). Fumonisin levels detected in field-grown maize have fluctuated widely depending on location and growing season, but both preharvest and postharvest surveys of field maize have indicated that the potential for high levels of fumonisins exists (Murphy P A, Rice L G, Ross P F (1993) “Fumonisin-B1, Fumonisin-B2, and Fumonisin-B3 content of Iowa, Wisconsin, and Illinois corn and corn screenings.”
J Agr Food Chem
41: 263-266). Surveys of food and feed products have also detected fumonisin (Holcomb M, Thompson H C Jr., Hankins L J (1993) “Analysis of fumonisin B-1 in rodent feed by gradient elution HPLC using precolumn derivation with FMOC and fluorescence detection.”
J Agr Food Chem
41: 764-767; Hopmans E C, Murphy P A (1993) “Detection of Fumonisin-B(1), Fumonisin-B(2), and Fumonisin-B(3) and hydrolyzed Fumonisin-B(1) in Corn-Containing foods.”
J Agr Food Chem
41: 1655-1658; Sydenham E W, Shephard G S, Thiel P G, Marasas W F O, Stockenstrom S (1991) “Fumonisin Contamination of Commercial Corn-Based Human Foodstuffs.”
J Agr Food Chem
39: 2014-2018). The etiology of Fusarium ear mold is poorly understood, although physical damage to the ear and certain environmental conditions can contribute to its occurrence (Nelson P E (1992) “Taxonomy and Biology of
Fusarium moniliforme.” Mycopathologia
117: 29-36). Fusarium can be isolated from most field grown maize, even when no visible mold is present. The relationship between seedling infection and stalk and ear diseases caused by Fusarium is not clear. Genetic resistance to visible kernel mold has been identified (Gendloff E, Rossman E, Casale W, Isleib T, Hart P (1986) “Components of resistance to Fusarium ear rot in field corn.”
Phytopathology
76: 684-688; Holley R N, Hamilton P B, Goodman M M (1989) “Evaluation of tropical maize germplasm for resistance to kernel colonization by
Fusarium moniliforme.” Plant Dis
73: 578-580), but the relationship to visible mold to fumonisin production has yet to be elucidated.
Fumonisins have been shown in in vitro mammalian cell studies to inhibit sphingolipid biosynthesis through inhibition of the enzyme sphinganine acyl transferase, resulting in the accumulation of the precursor sphinganine. (Norred W P, Wang E, Yoo H, Riley R T, Merrill A H (1992) “In vitro toxicology of fumonisins and the mechanistic implications.”
Mycopathologia
117: 73-78; Wang E, Norred W, Bacon C, Riley R, Merrill A Jr. (1991) “Inhibition of sphingolipid biosynthesis by fumonisins: implications for diseases associated with
Fusarium moniliforme.” J Biol Chem
266: 14486; Yoo H S, Norred W P, Wang E, Merrill A H, Riley R T (1992) “Fumonisin Inhibition of de Novo Sphingolipid Biosynthesis and Cytotoxicity Are Correlated in LLC-PK1 Cells.”
Toxicol Appl Pharmacol
114: 9-15) It is likely that inhibition of this pathway accounts for at least some of fumonisin's toxicity, and support for this comes from measures of sphinganine:sphingosine ratios in animals fed purified fumonisin (Wang E, Ross P F, Wilson T M, Riley R T, Merrill A H (1992) “Increases in Serum Sphingosine and Sphinganine and Decreases in Complex Sphingolipids in Ponies Given Feed Containing Fumonisins, Mycotoxins Produced by
Fusarium moniliforme.” J Nutr
122: 1706-1716). Fumonisins also affect plant cell growth (Abbas H K, Boyette C D (1992) “Phytotoxicity of fumonisin B
1
on weed and crop species.”
Weed Technol
6: 548-552; Vanasch M A J, Rijkenberg F H J, Coutinho T A (1992) “Phytotoxicity of fumonisin B
1
, moniliformin, and t-2 toxin to corn callus cultures.”
Phytopathology
82: 1330-1332; Vesonder R F, Peterson R E, Labeda D, Abbas H K (1992) “Comparative phytotoxicity of the fumonisins,
AAL
-Toxin and yeast sphingolipids in
Lemna minor
L. (Duckweed).”
Arch Environ Contam Toxicol
23: 464-467). Kuti et al. “Effect of fumonisin B1 on virulence of Fusarium species isolated from tomato plants.” (Abstract, Annual Meeting American Phytopathological Society, Memphis, Tenn.: APS Press 1993) reported on the ability of exogenously added fumonisins to accelerate disease development and increase sporulation of
Fusarium moniliforme
and
F. oxysporum
on tomato.
The toxicity of fumonisins and their potential widespread occurrence in food and feed makes it imperative to find detoxification or elimination strategies to remove the compound from the food chain.
DISCLOSURE OF THE INVENTION
The present invention provides newly discovered enzymes capable of degrading and detoxifying fumonisins, produced by fermentation of

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Fumonisin detoxification compositions and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fumonisin detoxification compositions and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fumonisin detoxification compositions and methods will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3104352

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.