Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1991-03-08
1993-12-21
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435226, 4352402, 43524021, C12N 1501, C12N 506
Patent
active
052720705
ABSTRACT:
Disclosed is the preparation of eukaryotic cells which are capable of producing smaller and less heterogenic N-linked glycans, particularly glycans having the structure Man.sub.3 GlcNAc.sub.2. In particular embodiments, CHO cells are produced having two particular defects in enzymes involved in N-linked glycosylation. The first defect, PIR, leads to an accumulation of Man.sub.5 GlcNAc.sub.2 -P-P-dolichol and thus lends resistance to glycosylation processing inhibitors such as castanospermine and swainsonine. The second defect involves a defect in the enzyme N-acetylglucosaminyltransferase I activity, and thus prevents the attachment of N-acetylglucosamine residues to oligosaccharide structures. About 70 to 75% of the N-linked glycans produced by cells of the present invention are thus in the form of Man.sub.3 GlcNAc.sub.2, making these lines particularly well suited to the production of proteins, e.g., recombinant proteins such as EPO and t-PA, which one desires to have a smaller and more homogeneous N-linked glycan structure.
REFERENCES:
patent: 4703008 (1987-04-01), Lin
Zeng & Lehrman, J Biol Chem, 265(4):2296-2305, 1990.
Takeuchi et al., J Biol Chem, 265(21):12127-12130, 1990.
Kumar & Stanley, Mol Cell Biol, 9(12):5713-5717, 1989.
Spellman et al., J Biol Chem, 264(24):14100-14111, 1989.
Lehrman & Zeng, J Biol Chem, 264(3):1584-1593, 1989.
Stanley, Mol Cell Biol, 9(2):377-383, 1989.
Stanley & Chaney, Mol Cell Biol, 5(6):1204-1211, 1985.
Chaney & Stanley, J Biol Chem, 261:10551-10557, 1986.
Stanley, Ann Rev Genet, 18:525-552, 1984.
Gottlieb et al., J BIOL Chem, 250(9):3303-3309, 1975.
Darnell et al., Molecular Cell Biology, Scientific American Books, Inc., pp. 957-964, 1986.
Lehrman Mark A.
Zeng Yucheng
Board of Regents , The University of Texas System
Ketter James
Schwartz Richard A.
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