Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1989-12-11
1994-01-04
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
424 92, 435 712, 530350, 530417, 530825, A61K 3910, C07K 302, C07K 320, C07K 1504
Patent
active
052761428
ABSTRACT:
A process for extracting and purifying a protein having an apparent molecular weight of about 69,000 daltons from the outer membrane of the bacterium Bordetella pertussis is provided. The process includes inactivating Bordetella pertussis cells with a bacteriostatic agent, repetitive extraction, and purification of the 69,000 dalton protein from extract by dye ligand chromatography followed by chromatofocusing. The process results in improved yield and stability of the 69,000 dalton protein.
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American Cyanamid Company
Russel Jeffrey E.
Szatkowski T. S.
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