Method for testing blood units for viral contamination

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 6, 435 71, 435 912, 435 2, 210789, 210782, 424 9372, 424 9374, C12Q 170, C12Q 168, B01D 3315, B01D 2126

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active

056630450

ABSTRACT:
The present invention provides a method for testing a blood unit for viral contamination without rendering the blood unit unusable for therapeutic applications. The method comprises the steps of: removing and collecting from a blood unit a majority of the leukocytes present therein; and using the collected leukocytes to test the blood unit for viral contamination. The present invention also provides a method for validating viral inactivation processes.

REFERENCES:
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Frewin et al, A Comparative Study of the Effect of Three Methods of Leukocyte Removal on Plasma Histamine Levels in Stored Human Blood, Seminars in Hematology, vol. 28, No. 3, pp. 18-21 (1991).
Barre-Sinoussi et al, Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS), Science, vol. 220, pp. 868-871 (1983).
Perrow, The Preparation of Leucocyte Homogenates for Enzymatic Assays, New Zealand Journal of Laboratory Techniques, vol. 31, No. 1, pp. 6-9 (1977).
Bruisten et al, Efficiency of White Cell Filtration and a Freeze-Thaw Procedure for Removal of HIV-infected Cells from Blood, Transfusions, vol. 30, No. 9, pp. 833-837 (1990).

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