Process for purifying DNA on hydrated silica

Details Process for purifying DNA on hydrated silica Process for purifying DNA on hydrated silica

1993-04-23

1994-08-30

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435274, 935 19, C12P 1934, C12Q 168, C07H 2100, C07H 2104

Patent

active

053429311

ABSTRACT:
A process for purifying DNA comprising 1) binding the DNA to a hydrated silica in the presence of water or physiological buffers in which the hydrated silica is prepared by refluxing silicon dioxide in sodium hydroxide or potassium hydroxide at a molar ratio of about 2:1 to 10:1 for at least about 48 hours, 2) separating and washing hydrated silica and the DNA bound thereto, and eluting the DNA from the hydrated silica in a heated physiological buffer or in heated water.

REFERENCES:
patent: 4822681 (1989-04-01), Schossler et al.
patent: 4833234 (1984-05-01), DeBonville et al.
patent: 4900677 (1990-02-01), Hewitt
patent: 4921952 (1990-05-01), Longmire et al.
patent: 4923978 (1990-05-01), McCormick
patent: 5075430 (1991-12-01), Little
patent: 5106966 (1992-04-01), Thomas et al.
patent: 5155018 (1992-10-01), Gillespie et al.
patent: 5175271 (1992-12-01), Thomas et al.
patent: 5234809 (1993-08-01), Boom et al.
R. Boom et al. J. Clin. Micro. 28(3):495-503, 1990.
M. A. Marko et al. Anal. Biochom. 121:382-387, 1982.
Erik H. Willis et al. Biotechniques 9(1):92-99, 1990.
Sek C. Chow et al., Analytical Biochemistry 183:42 (1989).
Randy M. McCormick Analytical Biochemistry 181:66 (1989).
Louise H. Lutze et al., Nucleic Acids Research 18:6150 (1990).
Bert Vogelstein et al., Proc. Natl. Acad. Sci. 76:615 (1979).

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