Process for demineralization of bone using column extraction

Details Process for demineralization of bone using column extraction Process for demineralization of bone using column extraction

1991-03-05

1994-01-04

Johnston, Jill A.

Chemistry: analytical and immunological testing

Metal or metal containing

Present in biological fluids

422 69, 422 70, 422261, 424549, 424572, 436 79, 436150, 436163, 436164, 436177, 436178, 530422, 530423, 530424, 530426, B01D 1102, G01N 118, G01N 2700, C07K 302

Patent

active

052759547

ABSTRACT:
A process and apparatus are disclosed for the demineralization of ground bone particles or pieces of cancellous or cortical bone. The apparatus includes multiple solution reservoirs which supply solutions to be pumped into one or more columns filled with bone samples to be demineralized. Solvent outflowing from the column(s) can be monitored for pH, calcium ion concentration or conductivity as a basis for determining when demineralization is complete. During the delipidization phase of processing, lipid solute can be monitored by photometry, or a small assay of the eluent can be dropped into deionized water to see whether a precipitate develops. Flow through the apparatus, including the types and amounts of solutions to be flowed through the apparatus, can be manually or computer controlled.

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Process Validation for Demineralization of Ground Human Bone Matrix; Jae Eun Lee & Lloyd Wolfinberger; Oct. 1990.
Validation of Processing Steps in Bone Demineralization; J. E. Lee, L. Wolfinbarger, Jr., S. Bottenfield, M. Conley, & M. John; Sep. 1990.

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