Method for quantifying oxidation parameters of low density lipop

Details Method for quantifying oxidation parameters of low density lipop Method for quantifying oxidation parameters of low density lipop

1997-03-25

1999-02-23

Snay, Jeffrey

Chemistry: analytical and immunological testing

Lipids, triglycerides, cholesterol, or lipoproteins

436172, 436178, G01N 2176, G01N 3392

Patent

active

058743138

ABSTRACT:
A method for the determination of the oxidizability of low density lipoproteins (LDL) in a serum or plasma sample from a mammal, which method comprises
isolating the LDL from the serum or plasma sample for the preparation of a LDL fraction,
separating the lipids from the LDL fraction to obtain a lipid fraction therefrom,
determining the baseline level of conjugated dienes (BDC) in the lipid fraction.

REFERENCES:
Wieland, H. et al "A simple specific method for precipitation of low density lipoproteins" Journal of Lipid Research, vol. 24 (1983) pp. 904-909.
Lissi, E. et al "Evaluation of total antioxidant potential (TRAP) and total antioxidant reactivity from luminol-enhanced chemiluminescence measurements" Chemical Abstracts, vol. 122 (1995) abstract No. 127879m.
Kujala UM, et al., "Low LDL oxidation in veteran endurance athletes," Scandinavian Journal of Medicine & Science in Sports, 1996.
D.D.M. Wayner, et al., "Quantitative measurement of the total, peroxyl radical-trapping antioxidant capability of human blood plasma by controlled peroxidation," FEBS Letters, 187: 33-37 (1985).
Juha Alanko, et al., "Modulation of Arachidonic Acid Metabolism By Phenols: Relation to Positions of Hydroxyl Groups and Peroxyl Radical Scavenging Properties," Radical Biology & Medicine, vol. 14, 1993.
H.A. Kleinveld, et al. "Improved Measurement of Low-Density-Lipoprotein Susceptibility to Copper-Induced Oxidation: Application of a Short Procedure for Isolating Low-Density Lipoprotein," Clin. Chem. 1992.
Vasankari, et al., "Measurement of serum lipid peroxidation during exercise using three different methods: diene conjugation, thiobarbituric acid reactive material and fluorescent chromolipids" CCA 234 (1995).

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