Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1991-12-20
1999-02-23
Prats, Francisco C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 72, 435100, 435101, 435105, 435193, 435815, C12P 1918, C12P 1912, C12P 1904, C12N 910
Patent
active
058742611
ABSTRACT:
A reiterative method for obtaining the glycosyltransferases useful for glycosyltransferase-catalyzed synthesis of a saccharide composition by serially bonding preselected saccharide units onto an immobilized acceptor moiety which is one member selected from the group consisting of proteins, glycoproteins, lipids, glycolipids and carbohydrates. The method comprises in a first iteration, the steps of (i) contacting an acceptor moiety immobilized onto a solid or semi-solid support with a mixture containing at least one glycosyltransferase under conditions effective to allow said glycosyltransferase to bind to the immobilized acceptor moiety thereby resulting in an immobilized acceptor moiety-glycosyltransferase complex, followed by (ii) contacting the immobilized acceptor moiety-glycosyltransferase complex with a preselected saccharide under conditions and in the presence of co-reagents sufficient to effect bonding of the preselected saccharide unit onto the immobilized acceptor moiety catalyzed by the glycosyltransferase thereby freeing the glycosyltransferase from the complex and obtaining an immobilized product. This is followed by a single or a plurality of subsequent iterations of steps (i) and (ii), such that the immobilized product obtained in step (ii) of a given iteration is used as the immobilized acceptor moiety in step (i) of the following iteration.
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Prats Francisco C.
The Trustees of the University of Pennsylvania
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