Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Reexamination Certificate
2000-02-04
2002-06-11
Warden, Jill (Department: 1743)
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
C422S051000, C422S105000, C422S063000, C422S105000, C422S082050, C422S082120, C436S180000, C436S172000, C435S288700, C435S288500, C356S340000, C250S238000
Reexamination Certificate
active
06403037
ABSTRACT:
FIELD OF THE INVENTION
This invention relates to systems and methods for chemical analysis, and in particular to a novel reaction vessel and temperature control system.
BACKGROUND OF THE INVENTION
There are many applications in the field of chemical processing in which it is desirable to precisely control the temperature of a biological sample, to induce rapid temperature changes in the sample, and to detect target analytes in the sample. Applications for such heat-exchanging chemical reactions may encompass organic, inorganic, biochemical or molecular reactions. Examples of thermal chemical reactions include isothermal nucleic acid amplification, thermal cycling nucleic acid amplification, such as the polymerase chain reaction (PCR), ligase chain reaction (LCR), self-sustained sequence replication, enzyme kinetic studies, homogeneous ligand binding assays, and more complex biochemical mechanistic studies that require complex temperature changes. Temperature control systems also enable the study of certain physiologic processes where a constant and accurate temperature is required.
One of the most popular uses of temperature control systems is for the performance of PCR to amplify a segment of nucleic acid. In this well known methodology, a DNA template is used with a thermostable DNA polymerase, nucleoside triphosphates, and two oligonucleotides with different sequences, complementary to sequences that lie on opposite strands of the template DNA and which flank the segment of DNA that is to be amplified (“primers”). The reaction components are cycled between a higher temperature (e.g., 95° C.) for dehybridizing double stranded template DNA, followed by lower temperatures (e.g., 40-60° C. for annealing of primers and 70-75° C. for polymerization). Repeated cycling between dehybridization, annealing, and polymerization temperatures provides exponential amplification of the template DNA.
Nucleic acid amplification may be applied to the diagnosis of genetic disorders; the detection of nucleic acid sequences of pathogenic organisms in a variety of samples including blood, tissue, environmental, air borne, and the like; the genetic identification of a variety of samples including forensic, agricultural, veterinarian, and the like; the analysis of mutations in activated oncogenes, detection of contaminants in samples such as food; and in many other aspects of molecular biology. Polynucleotide amplification assays can be used in a wide range of applications such as the generation of specific sequences of cloned double-stranded DNA for use as probes, the generation of probes specific for uncloned genes by selective amplification of particular segments of cDNA, the generation of libraries of cDNA from small amounts of mRNA, the generation of large amounts of DNA for sequencing and the analysis of mutations.
A preferred detection technique for chemical or biochemical analysis is optical interrogation, typically using fluorescence or chemiluminescence measurements. For ligand-binding assays, time-resolved fluorescence, fluorescence polarization, or optical absorption is often used. For PCR assays, fluorescence chemistries are often employed.
Conventional instruments for conducting thermal reactions and for optically detecting the reaction products typically incorporate a block of metal having as many as ninety-six conical reaction tubes. The metal block is heated and cooled either by a Peltier heating/cooling apparatus or by a closed-loop liquid heating/cooling system in which liquid flows through channels machined into the block. Such instruments incorporating a metal block are described in U.S. Pat. No. 5,038,852 to Johnson and U.S. Pat. No. 5,333,675 to Mullis.
These conventional instruments have several disadvantages. First, due to the large thermal mass of a metal block, the heating and cooling rates in these instruments are limited to about 1° C./sec resulting in longer processing times. For example, in a typical PCR application, fifty cycles may require two or more hours to complete. With these relatively slow heating and cooling rates, some processes requiring precise temperature control are inefficient. For example, reactions may occur at the intermediate temperatures, creating unwanted and interfering side products, such as PCR “primer-dimers” or anomalous amplicons, which are detrimental to the analytical process. Poor control of temperature also results in over-consumption of expensive reagents necessary for the intended reaction.
A second disadvantage of these conventional instruments is that they typically do not permit real-time optical detection or continuous optical monitoring of the chemical reaction. For example, in conventional thermal cycling instruments optical fluorescence detection is typically accomplished by guiding an optical fiber to each of ninety-six reaction sites in a metal block. A central high power laser sequentially excites each reaction site and captures the fluorescence signal through the optical fiber. Since all of the reaction sites are sequentially excited by a single laser and since the fluorescence is detected by a single spectrometer and photomultiplier tube, simultaneous monitoring of each reaction site is not possible.
Some of the instrumentation for newer processes requiring faster thermal cycling times has recently become available. One such device is disclosed by Northrup et al. in U.S. Pat. No. 5,589,136. The device includes a silicon-based, sleeve-type reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The device optionally includes a secondary tube (e.g., plastic) for holding the sample. In operation, the tube containing the sample is inserted into the silicon sleeve. Each sleeve also has its own associated optical excitation source and fluorescence detector for obtaining real-time optical data. This device permits faster heating and cooling rates than the instruments incorporating a metal block described above. There are, however, several disadvantages to this device in its use of a micromachined silicon sleeve. A first disadvantage is that the brittle silicon sleeve may crack and chip. A second disadvantage is that it is difficult to micromachine the silicon sleeve with sufficient accuracy and precision to allow the sleeve to precisely accept a plastic tube that holds the sample. Consequently, the plastic tube may not establish optimal thermal contact with the silicon sleeve.
SUMMARY
The present invention overcomes the disadvantages of the prior art by providing an improved instrument and reaction vessel for thermally controlling and optically interrogating a sample. In contrast to the prior art instruments described above, the system of the present invention permits extremely rapid heating and cooling of the sample, ensures optimal thermal transfer between the sample and heating or cooling elements, and provides for real-time optical detection and monitoring of the sample with increased detection sensitivity.
In a preferred embodiment, the system of the present invention includes a reaction vessel for holding a sample for chemical reaction and optical detection. The vessel has a rigid frame defining the side walls of a reaction chamber, and at least one flexible sheet attached to the rigid frame to form a major wall of the chamber. The vessel also includes a loading structure extending from the frame for loading a sample into the chamber. The loading structure defines a loading reservoir in fluid communication with the chamber. The loading reservoir receives the sample prior to loading the sample into the chamber. The loading structure also includes an aspiration port in fluid communication with the chamber.
The system also includes an aspiration and dispensing device, such as a pipette or syringe, for dispensing the sample into the loading reservoir, for subsequently establishing a seal with the aspiration port, and for drawing the sample from the loading reservoir into the chamber by vacuum. Loading the sample into the chamber in this manner reduces the likelihoo
Chang Ronald
Christel Lee A.
Dority Douglas B.
Petersen Kurt E.
Cepheid
Gordon Brian R
Townsend and Townsend / and Crew LLP
Warden Jill
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