Method of retrograde fluorescent labeling of neurons

Details Method of retrograde fluorescent labeling of neurons Method of retrograde fluorescent labeling of neurons

1985-09-25

1988-01-05

Rosenbaum, C. Fred

Surgery

Truss

Pad

514636, 424 9, 128 1R, A61B 505

Patent

active

047169052

ABSTRACT:
A fluorescent retrograde axonal tracer method for use in neuroanatomical studies of the brain involves the use of dilute solutions of amidino-stilbene compounds or salts, specifically 2-hydroxy-4,4'-diamidinostilbene isethionate. The amidino stilbene tracer exhibits a high sensitivity and fluorescent intensity, retrogradely fills axons and dendrites extensively, is highly resistant to fading, is compatible with virtually all other histochemical procedures, and exhibits minimal uptake by fibers of passage. The method of using amidino stilbene or stilbamadine compounds involves injection of a dilute solution of the compound into the area of the brain to be studied. After a period of time, the tissue is examined under a fluorescence microscope.

REFERENCES:
patent: 2510047 (1950-05-01), Ewins
Murgatrayd, 2 Hydroxystilbamidine Isethionate: A New Fluorochrome for Use in General Pathology in Histochemistry, 74(1), 1982, pp. 107-114.
L. C. Schmued, et al., SITS: A Covalently Bound Fluorescent Retrograde Tracer That Does Not Appear to be Taken Up by Fibers-of-Passage, Brain Research, 249, (1982), pp. 137-141.
M. Bentivolgio, et al., Fluorescent Retrograde Triple Labeling of Brainstem Reticular Neurons, Neuroscience Letters, 46, (1984), pp. 121-126.
H. G. J. M. Kuypers, et al., Retrograde Transport of Bisbenzimide and Propidium Iodide Through Azons to Their Parent Cell Bodies, Neuroscience Letters, 12, (1979), pp. 1-7.
M. Bentivoglio, et al., Fluorescent Retrograde Neuronal Labeling in Rat by Menas of Substances Binding Specifically to Adeninethymine Rich DNA, Neuroscience Letters, 12, (1979), pp. 235-240.
M. Bentivoglio, et al., Two New Fluorescent Retrograde Neuronal Tracers which are Transported Over Long Distances, Neuroscience Letters, 18, (1980), pp. 25-30.
M. Bentivoglio, et al., Retrograde Neuronal Labeling by Means of Bisbenzimide and Nuclear Yellow (Hoechst S 769121). Measures to Prevent Diffusion of the Tracers Out of Retrogradely Labeled Neurons, Neuroscience Letters, 18, (1980), pp. 19-24.
H. G. J. M. Kuypers, et al., Double Retrograde Neuronal Labeling Through Divergent Axon Collaterals, Using Two Fluorescent Tracers with the Same Excitation Wavelength which Label Different Features of the Cell, Brain Research, (1980), pp. 383-392.
K. Keizer, et al., Diamidino Yellow Dihydrochloride (KY-2HCI); New Fluorescent Retrograde Neuronal Tracer, Which Migrates Only Very Slowly Our of the Cell., Exp. Brain Research, 51, (1983), pp. 179-191.
L. C. Katz, et al., Fluorescent Latex Microspheres as a Retrograde Neuronal Marker for in Vivo and in Vitro Studies of Visual Cortex, Letters to Nature, vol. 310, Aug. 1984, pp. 498-500.

Affiliated with

Also associated with

Rating

Method of retrograde fluorescent labeling of neurons does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of retrograde fluorescent labeling of neurons, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of retrograde fluorescent labeling of neurons will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2893