Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
1999-11-05
2002-04-02
Fox, David T. (Department: 1638)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S468000, C435S320100, C435S419000, C800S278000, C800S298000
Reexamination Certificate
active
06365728
ABSTRACT:
FIELD OF THE INVENTION
The present invention is directed to nucleic acid sequences that control the expression of genes in eukaryotic cells. More particularly, the invention is directed to a gene promoter that confers a high level of expression to genes that are operably linked to the promoter.
BACKGROUND AND SUMMARY OF THE INVENTION
The present invention relates to a novel regulatory element which confers a high level of expression in plant cells to genes that are operably linked to the regulatory element. The ability to control the level of gene expression in plants is important for many applications of genetic transformation procedures including those directed to crop improvement.
In eukaryotic organisms, multi-level regulatory systems exist to control gene expression. The transcription process is an integral part of such systems and is involved in synthesis of mRNA molecules. The efficiency of transcription is mostly determined by a region of DNA called the promoter. The promoter consists of gene sequences upstream of the site of transcription initiation. The components of the promoter region include the “TATA” box and often a “CAAT” box. In addition, many other regulatory elements that affect transcription may be present in the promoter sequences. The coordinated action of cellular proteins (transcription factors) interacting with promoter sequences determines the specificity of a particular promoter and its effectiveness. Since most eukaryotic genes are stringently regulated, there is a limited availability of promoters with constitutive, strong expression.
The present invention describes the isolation and purification of a DNA sequence that expresses operably linked genes to high levels in plant cells. The promoter sequence described in the present invention expresses genes at a level equal to or higher than that obtained from one of the strongest presently available promoters —the 35S cauliflower mosaic virus promoter. Such promoters are needed to direct a high level of protein expression in transgenic plants. The strong promoter of the resent invention is used to construct expression vectors for expressing genes in plant cells. In one embodiment, a plant expression vector is provided that comprises the regulatory element of SEQ ID NO: 2 operably linked to a non-natively associated gene, and this vector is used to produce transgenic plants.
REFERENCES:
patent: WO 97/13401 (1997-04-01), None
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Yoshida, T. et al., GenEmbl, Accession No. D63789, Aug. 07, 1995.*
Fisscher, et al., “Identification of potential regulatory elements in the far-upstream region of theArabidopsis thalianaplastocyanin promoter.” Nov. 1994,Plant Molecular Biology, vol. 26, No. 3, pp. 873-886.
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Lazar, et al., “Identification of Plant Serine-Arginine-Rich Protein Similar to the Mammalian Splicing Factor SF2/ASF.” Aug. 1995,Proc. Natl. Acad. Sci. USA, vol.92, pp. 7672-7676.
Lopato, et al., “Characterization of a Novel Arginine-Serine-Rich Splicing Factor in Arabidopsis.” Dec. 1996,Plant Cell, vol. 8, No. 12, pp. 2255-2264.
Su, et al., “Arabidopsis ASF-SF2 Transcripts are Alternatively Spliced.” Jul. 1997,Plant Physiology, vol. 114, No. 3, p. 246.
Hodges Thomas K.
Lyznik Leszek A.
Barnes & Thornburg
Fox David T.
Kubelik Anne R
Purdue Research Foundation
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