Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2008-07-29
2008-07-29
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S252300
Reexamination Certificate
active
07405059
ABSTRACT:
Novel Shine-Dalgarno (ribosome binding site) sequences, vectors containing such sequences, and host cells transformed with these vectors are provided. Methods of use of such sequences, vectors, and host cells for the efficient production of proteins and fragments thereof in prokaryotic systems are also provided. In particular embodiments of the invention, compounds and methods for high efficiency production of soluble protein in prokaryotic systems are provided.
REFERENCES:
patent: 3979506 (1976-09-01), Smith
patent: 4358595 (1982-11-01), Ghosh et al.
patent: 4582789 (1986-04-01), Sheldon, III et al.
patent: 5109124 (1992-04-01), Ramachandran et al.
patent: 5317098 (1994-05-01), Shizuya et al.
patent: 5663319 (1997-09-01), Bittner et al.
patent: 6096545 (2000-08-01), LeFebvre et al.
patent: 6194168 (2001-02-01), Gentz et al.
patent: 2002/0039588 (2002-04-01), Collier et al.
patent: 2002/0048590 (2002-04-01), Klimpel et al.
patent: 2002/0051791 (2002-05-01), Galloway et al.
patent: WO99/16858 (1999-04-01), None
patent: WO00/56883 (2000-09-01), None
patent: WO01/82788 (2001-11-01), None
Chauhan, V. et al., “Constitutive expression of protective antigen gene ofBacillus anthracisinEscherichia coli,” Biochem. Biophys. Res. Commun.283(2):308-315 (2001).
Gupta, P. et al., “Expression and purification of the recombinant protective antigen ofBacillus anthracis,” Protein Expr. Purif.7:33-38 (1996).
Komarova et al., “Extensive complementarity of the Shine-Dalgarno region and 3′-terminal sequence of 16S ribosomal RNA is inefficient for translation in vivo,”Bioorg. Khim.27(4):282-290 (2001) (in Russian; abstract in English).
Sellman, B.R. et al., “Point mutations in anthrax protective antigen that blocks translocation,”J. Biol. Chem.276(11):8371-8376 (2001).
Sharma, M. et al., “Expression and purification of anthrax protective antigen fromEscherichia coli,” Protein Expr. Purif.16(3):369-376 (1999).
Shine & Dalgarno, “The 3′-Terminal Sequence ofEscherichia coli16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites,”Proc. Natl. Acad. Sci. USA71(4):1342-1346 (1976).
Stenström et al., “Codon bias at the 3′-side of the initiation codon is correlated with translation initiation efficiency inEscherichia coli,” Gene263(1-2):273-284 (2001).
Stenström et al., “Cooperative effects by the initiation codon and its flanking regions on translation initiation,”Gene273(2):259-265 (2001).
Kammerer et al., “Functional dissection ofEscherichia colipromoters: information in the transcribed region is involved in late steps of the overall process,”EMBO J.5(11):2995-3000 (1986).
Pearce, GenEmbl Database, Accession No. AL 160036, Sep. 30, 2000.
Zhao et al., EST Database, Accession No. AZ102942, May 9, 2000.
Supplementary European Search Report, European Application No. EP 03 76 1989, mailed Sep. 16, 2005.
Human Genome Sciences Inc.
Vogel Nancy
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