Stabilized polynucleotide complexes and methods

Details Stabilized polynucleotide complexes and methods Stabilized polynucleotide complexes and methods

2008-01-29

2008-01-29

Marschel, Ardin H. (Department: 1614)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C514S04400A

Reexamination Certificate

active

07323297

ABSTRACT:
The invention comprises stabilized polynucleotide complexes that have a cryoprotectant and are lyophilized. Cryoprotectant compounds comprise carbohydrates or sugars, preferably lactose and sucrose, but also glucose, maltodextrins, mannitol, sorbitol, trehalose, and others. Other suitable cryoprotectants include amino acids such as betaines and prolines. Polynucleotide complexes stabilized according to the invention can be used for transfection, and exhibit improved tranfection efficiency with respect to polynucleotide complexes without cryoprotection.

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