Propagation and/or derivation of embryonic stem cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

Reexamination Certificate

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C435S455000

Reexamination Certificate

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07371573

ABSTRACT:
Embryonic stem (ES) cells are cultured in the presence of a compound which selectively inhibits propagation or survival of cells other than ES cells. The ES cells have not been genetically altered. Instead, the compound inhibits a signalling pathway which is essential for propagation of differentiated cells but is not essential for propagation of ES cells—hence ES cells are selectively maintained in the culture.

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