Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Reexamination Certificate
2008-03-18
2008-03-18
Lucas, Zachariah (Department: 1648)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
C530S350000, C530S351000, C530S402000, C424S085100, C435S069100, C435S069400, C435S069500, C514S002600
Reexamination Certificate
active
07345154
ABSTRACT:
The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby.
REFERENCES:
patent: 4588585 (1986-05-01), Mark et al.
patent: 5166322 (1992-11-01), Shaw et al.
patent: 5206344 (1993-04-01), Katre et al.
patent: 5208158 (1993-05-01), Bech et al.
patent: 5766897 (1998-06-01), Braxton
patent: 5849535 (1998-12-01), Cunningham et al.
patent: 5916773 (1999-06-01), Mele et al.
patent: 0 458 064 (1991-11-01), None
patent: WO 90/12874 (1990-11-01), None
patent: WO 93/00109 (1993-07-01), None
patent: WO 95/11987 (1995-04-01), None
patent: WO 95/32003 (1995-11-01), None
patent: WO 96/31537 (1996-10-01), None
Lin et al., “Cloning and expression of the human erythropoietin gene,” Proceedings of the National Academy of Sciences, USA, vol. 82 No. 22, pp. 7580-7584 (Nov. 1985).
Nielson et al., “Erythropoietin-beta-D-galactosidase. The generation, purification and use of a fusion protein,” Journal of Immunological Methods, vol. 111 No. 1, pp. 1-9 (Jun. 1988).
Weich et al., “Interleukin-3/erythropoietin fusion proteins: in vitro effects on hematopoietic cells,” Experimental Hematology, vol. 21 No. 5, pp. 647-655 (May 1993).
Bil et al.,Biochimica et Biophysica Acta, 1261: 34-43 (1995).
Bittorf et al.,Febs. Lett., 366: 133-136 (1993).
Cheetham et al.,Nat. Structural Biol., 5: 861-866 (1998).
Dube et al.,The Journal of Biol. Chem., vol. 263, No. 33: 17516-17521 (1988).
Elliot et al.,Blood, 89:493-502 (1997).
Matthews et al.,PNAS, 93: 9471-9476 (1996).
Syed et al.,Nature, 395:511-516, (1998).
Wen et al.,The Journal of Biol. Chem., vol. 269, No. 36: 22839-22846 (1994).
Bazan,Immunology Today, 11:350-354 (1990).
Boissel et al.,J. Biol. Chem., 268(21):15983-15993 (1993).
Campbell et al.,J. Peptide Res., 49:527-537 (1997).
Cunningham et al.,Science, 243:1330-1336 (1989).
Goodson and Katre,Bio/Technology, 8:343-346 (1990).
Karpusas et al.,Proc. Natl. Acad. Sci. USA, 94:11813-11818 (1997).
Lowman and Wells,J. Mol. Biol., 234:564-578 (1993).
Mott and Compbell,Curr Opin Struct Biol, 5:114-121 (1995).
Sprang and Bazan,Curr. Op. Struct. Biol., 3:815-827 (1993).
Wells,Ann. Rev. Biochem., 65:609-634 (1996).
Zalipsky, S.,Adv. Drug Delivery Rev., 16:157-182 (1995).
Bolder Biotechnology Inc.
Lucas Zachariah
Sheridan & Ross P.C.
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