Method for collecting and using nuclear mRNA

Details Method for collecting and using nuclear mRNA Method for collecting and using nuclear mRNA

2008-05-20

2008-05-20

Benzion, Gary (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

07374881

ABSTRACT:
The present invention is related to a novel method of detecting mRNA, based on the fact that the newly synthesized mRNA is confined inside the cell nucleus. According to the novel method cells are captured on a membrane and then treated by a cell membrane permeation solution, thereby increasing permeability of cell membranes. After the cytoplasm is washed away, nuclei are dissolved with a cell dissolving solution, and then mRNA can be successfully recovered from the obtained solution.

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