Process for the extraction, purification and enzymatic...

Details Process for the extraction, purification and enzymatic... Process for the extraction, purification and enzymatic...

2011-03-08

2011-03-08

Tate, Christopher R (Department: 1655)

Drug, bio-affecting and body treating compositions

Plant material or plant extract of undetermined constitution...

Containing or obtained from leguminosae

Reexamination Certificate

active

07901718

ABSTRACT:
A process for the extraction, purification and enzymatic modification of β-conglycinin α′ subunit, wherein β-conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the α′ subunit, which is treated with chymotrypsin, then subjected to a further MAC to recover the amino-terminal region of this polypeptide (MW 28,000 Da).

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