Method of collecting highly pure polyhydroxyalkanoate from...

Details Method of collecting highly pure polyhydroxyalkanoate from... Method of collecting highly pure polyhydroxyalkanoate from...

2008-07-01

2008-07-01

Lilling, Herbert J (Department: 1657)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S183000

Reexamination Certificate

active

07393668

ABSTRACT:
The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles.The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.

REFERENCES:
patent: 4358583 (1982-11-01), Walker et al.
patent: 4477654 (1984-10-01), Holmes et al.
patent: 5213976 (1993-05-01), Blauhut et al.
patent: 6808907 (2004-10-01), Honma et al.
patent: 0046017 (1982-02-01), None
patent: 55-118394 (1980-09-01), None
patent: 57-65193 (1982-04-01), None
patent: 63-198991 (1988-08-01), None
patent: 2-69187 (1990-03-01), None
patent: 5-507410 (1993-10-01), None
patent: 5-336982 (1993-12-01), None
patent: 7-31487 (1995-02-01), None
patent: 07-031487 (1995-02-01), None
patent: 7-31488 (1995-02-01), None
patent: 7-31489 (1995-02-01), None
patent: 07-031489 (1995-02-01), None
patent: 7-79788 (1995-03-01), None
patent: 4-61638 (1995-07-01), None
patent: 7-509131 (1995-10-01), None
patent: 8-502415 (1996-03-01), None
patent: 11-266891 (1999-10-01), None
patent: 2001-046094 (2001-02-01), None
patent: 20001-46094 (2001-02-01), None
patent: 2001-57895 (2001-03-01), None
patent: 2001-057895 (2001-03-01), None
patent: WO 92/22659 (1992-12-01), None
Harrison et al “Combined chemical and mechanical processes for the disruption of bacteria” Bioseparaton 2: pp. 95-105.
Jong-il Choi et al.: Efficient and Economic Recovery of poly(3-Hydroxybutyrate) from recombinantEscherichia coliby simple digestion with chemicals, Biotechnol. Bioeng. (1999), vol. 62, No. 5, pp. 546 to 553.

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