Production of proteins in active forms

Details Production of proteins in active forms Production of proteins in active forms

1988-02-11

1990-10-30

Moskowitz, Margaret

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Lymphokines, e.g., interferons, interlukins, etc.

435 693, 435 694, 435 6951, 435 695, 530408, 530409, 530350, 530399, 530420, 530422, 530423, 530412, 530404, 530405, C07K 1526, C07K 300

Patent

active

049669630

ABSTRACT:
A method for the preparation of proteins in biologically active form including providing a source of protein solubilized from inclusion bodies with a cationic surfactant; providing a weak denaturing agent; and contacting the solubilized protein with the weak denaturant in water in an amount sufficient to allow the protein to remain in a biologically active form.

REFERENCES:
patent: 4731440 (1988-03-01), Bentle et al.
patent: 4797474 (1989-01-01), Patroni et al.
Osterman, Lev A., Methods of Protein and Nucleic Acid Research, vol. 1, Springer-Verlag, New York, 1984.

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