Mutant firefly luciferase, gene, recombinant vector,...

Details Mutant firefly luciferase, gene, recombinant vector,... Mutant firefly luciferase, gene, recombinant vector,...

2011-08-23

2011-08-23

Kim, Alexander (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C536S023200

Reexamination Certificate

active

08003350

ABSTRACT:
The present invention provides a mutant firefly luciferase consisting of a mutant amino acid sequence derived from the amino acid sequence of a wild-type firefly luciferase by at least substitution (a), (b), or (c) below and having luminescence intensity higher than that of the wild-type firefly luciferase. According to the present invention, a mutant firefly luciferase with increased luminescence intensity compared with that of wild-type firefly luciferases is provided, regarding which (a) through (c) are as follows:(a) substitution of at least one of the amino acids at positions equivalent to positions 419 to 428 in the amino acid sequence of North American firefly luciferase with a nonpolar amino acid having a molecular weight that is the same as or higher than that of the amino acid to be substituted;(b) substitution of at least one of the amino acids at positions equivalent to positions 435 to 441 in the amino acid sequence of North American firefly luciferase with an amino acid that has a molecular weight lower than that of the amino acid to be substituted and is selected from among glycine, alanine, and serine; and(c) substitution of at least one of the amino acids at positions equivalent to positions 523 to 532 in the amino acid sequence of North American firefly luciferase with a positively-charged amino acid that has an isoelectric point higher than that of the amino acid to be substituted.

REFERENCES:
patent: 6812012 (2004-11-01), Hattori et al.
patent: 2004/0235077 (2004-11-01), Hattori et al.
patent: 3-285683 (1991-12-01), None
patent: 2666561 (1997-06-01), None
patent: 11-239493 (1999-09-01), None
patent: 2003-518912 (2003-06-01), None
patent: 00/24878 (2000-05-01), None
Guo et al., Protein tolerance to random amino acid change, 2004, Proc. Natl. Acad. Sci. USA 101: 9205-9210.
Lazar et al., Transforming Growth Factor α: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activity, 1988, Mol. Cell. Biol. 8:1247-1252.
Hill et al., Functional Analysis of conserved Histidines in ADP-Glucose Pyrophosphorylase fromEscherichia coli, 1998, Biochem. Biophys. Res. Comm. 244:573-577.
White et al., Improved thermostability of the North American firefly luciferase: saturation mutagenesis at position 354., Biochem. J., 1996, vol. 319, pp. 343-350.
Amino Acid Structure (last viewed on Nov. 11, 2010).
B. R. Branchini et al., “The Role of Lysine 529, A Conserved Residue of the Acyl-Adenylate-Forming Enzyme Superfamily, in Firefly Luciferase”, Biochemistry, vol. 39, No. 18, pp. 5433-5440, 2000.
B. R. Branchini et al., “Site-Directed Mutagenesis of Firefly Luciferase Active Site Amino Acids: A Proposed Model for Bioluminescence Color”, Biochemistry, vol. 38, No. 40, pp. 13223-13230, 1999.
B. R. Branchini et al., “Mutagenesis Evidence that the Partial Reactions of Firefly Bioluminescence are Catalyzed by Different Conformations of the Luciferase C-Terminal Domain”, Biochemistry, vol. 44, No. 5, pp. 1385-1393, 2005.
K. Ayabe et al., “The Role of Firefly Luciferase C-Terminal Domain in Efficient Coupling of Adenylation and Oxidative Steps”, FEBS Letters, vol. 579, pp. 4389-4394, 2005.
J. P. Waud et al., “Engineering the C-Terminus of Firefly Luciferase as an Indicator of Covalent Modification of Proteins”, Biochimica et Biophysica Acta, vol. 1292, pp. 89-98, 1996.

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