Digital holographic microscope for 3D imaging and process...

Details Digital holographic microscope for 3D imaging and process... Digital holographic microscope for 3D imaging and process...

2008-04-22

2008-04-22

Chowdhury, Tarifur (Department: 2886)

Optics: measuring and testing

By light interference

For dimensional measurement

Reexamination Certificate

active

07362449

ABSTRACT:
The present invention is related to a compact microscope able to work in digital holography for obtaining high quality 3D images of samples, including fluorescent samples and relatively thick samples such as biological samples, said microscope comprising illumination means (1,41) at least partially spatially coherent for illuminating a sample (2) to be studied and a differential interferometer (5) for generating interfering beams from said sample (2) on the sensor (33) of an electronic imaging device(7), said interferometer (5) comprising namely tilting means (17) for tilting by a defined angle one the interfering beams (28or29) relatively to the other, said tilting resulting into a defined shift (27) of said interfering beam on the sensor of the electronic imaging device (7), said shift (27) being smaller than spatial coherence width of each beam, said microscope being able to be quasi totally preadjusted independently from the samples so that minimum additional adjustments are required for obtaining reliable 3D images of samples.

REFERENCES:
patent: 3556660 (1971-01-01), Mottier
patent: 4165938 (1979-08-01), Doyle
patent: 844995 (1981-07-01), None
patent: WO 03/002972 (2003-01-01), None
Dubois et al., “Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence,”Applied Optics, vol. 38, No. 34, pp. 7085-7094, Dec. 1, 1999.
Pretzler et al., “High-accuracy differential interferometry for the investigation of phase objects,”Measurement Science and Technology, vol. 4, No. 6, pp. 649-658, Jun. 1, 1993.
Indebetouw et al., “Spatiotemporal digital microholography,”J. Opt. Soc. Am. A., vol. 18, No. 2, pp. 319-325, Feb. 2001.

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