Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2011-07-26
2011-07-26
Nashed, Nashaat (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S157000, C435S252300, C435S252330, C435S254110, C435S254210, C435S257200, C435S325000
Reexamination Certificate
active
07985567
ABSTRACT:
The invention provides for a method for producing a 5-carbon alcohol in a genetically modified host cell. In one embodiment, the method comprises culturing a genetically modified host cell which expresses a first enzyme capable of catalyzing the dephosphorylation of an isopentenyl pyrophosphate (IPP) or dimethylallyl diphosphate (DMAPP), such as aBacillus subtilisphosphatase (YhfR), under a suitable condition so that 5-carbon alcohol is 3-methyl-2-buten-1-ol and/or 3-methyl-3-buten-1-ol is produced. Optionally, the host cell may further comprise a second enzyme capable of reducing a 3-methyl-2-buten-1-ol to 3-methyl-butan-1-ol, such as a reductase.
REFERENCES:
patent: 4609624 (1986-09-01), Rothlisberger
patent: 5460949 (1995-10-01), Saunders et al.
patent: 6190895 (2001-02-01), Croteau et al.
patent: 6291745 (2001-09-01), Meyer et al.
patent: 6515202 (2003-02-01), Crane et al.
patent: 6531303 (2003-03-01), Millis et al.
patent: 6689593 (2004-02-01), Millis et al.
patent: 2003/0148479 (2003-08-01), Keasling et al.
patent: 2003/0219798 (2003-11-01), Gokarn et al.
patent: 2004/0005678 (2004-01-01), Keasling et al.
patent: 2004/0029239 (2004-02-01), Ohto et al.
patent: 2004/0063182 (2004-04-01), Ohto et al.
patent: 2004/0077039 (2004-04-01), Holtzman et al.
patent: 2004/0110259 (2004-06-01), Baugh et al.
patent: 2004/0194162 (2004-09-01), Hahn et al.
patent: 2007/0087425 (2007-04-01), Ohto
Chang et al, Production of isoprenoid pharmaceuticals by engineered microbes, Nature Chem Biology, (online) Nov. 15, 2006, pp. 1-8.
Keasling et al, 5-Carbon Alcohols for Drop-in Gasoline Replacement, LBNL Technology Announcement, posted online Sep. 2, 2009.
Martin et al, Engineering a mevalonate pathway inEscherichia colifor production of terpenoids, Nature Biotechnology (Jul. 2003), vol. 21, No. 7, pp. 796-802.
Maury et al, Microbial Isoprenoid Production: An Example of Green Chemistry through Metabolic Engineering, Adv Biochem Engin/Biotechnol (2005) 10: 19-51.
Rigden et al, A cofactor-dependent phosphoglycerate mutase homolog fromBacillus stearothermophilusis actually a broad specificity phosphatase. Protein Science 2001, 10:1835-1846; p. 1841, col. 2; p. 1842.
Smolenskii et al, a Study of the Structure-Octane Number Relationship for Hydrocarbons, Doklady Physical Chemistry, vol. 397, Part 1, 2004, pp. 145-149. Translated from Doklady Akademii Nauk, vol. 397, No. 2, 2004, pp. 219-224.
Withers et al, Identification of Isopentenol Biosynthetic Genes fromBacillus subtilisby a Screening Method Based on Isoprenoid Precursor Toxicity, Applied and Environmental Microbiology, Oct. 2007, pp. 6277-6283.
Polakowski et al, Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast, Appl Microbiol Biotechnol (1998) 49: 66-71.
Wilding et al, Identification, Evolution, and Essentiality of the Mevalonate Pathway for Isopentenyl Diphosphate Biosynthesis in Gram-Positive Cocci. J.Bacteriology, Aug. 2000, vol. 182, No. 15, pp. 4319-4327.
Donald et al, Effects of Overproduction of the Catalytic Domain of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase on Squalene Synthesis inSaccharomyces cerevisiae. Appl Env Microbiology, Sep. 1997, vol. 63, No. 9, pp. 3341-3344.
Jackson et al, Metabolic Engineering to Produce Sesquiterpenes in Yeast, Organ.Lett (2003) 5:1629-1632.
Hamano et al, Cloning of a Gene Cluster Encoding Enzymes Responsible for the Mevalonate Pathway from a Terpenoid-antibiotic-producing Streptomyces Strain. Biosci. Biotechnol. Biochem., (2001), 65 (7), 1627-1635.
Kuzuyama et al. Heterologous Mevalonate Production in Streptomyces lividans TK23. Biosci. Biotechnol. Biochem. (2004), 68 (4), 931-934.
Kazuhiko et al. Production of mevalonate by a metabolically-engineeredEscherichia coli. Biotechnology Lett (2004), vol. 26: 1487-1491.
Brock et al., On the mechanism of action of the antifungal agent propionate Propionyl-CoA inhibits glucose metabolism inAspergillus nidulans., Eur. J. Biochem. 271, 3227-3241 (2004).
Choi et al, High-Level Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Fed-Batch Culture of RecombinantEscherichia coli. Appl. Env. Microbiol. (Oct. 1999), vol. 65, No. 10, pp. 4363-4368.
Parke et al, Toxicity Caused by Hydroxycinnamoyl-Coenzyme A Thioester Accumulation in Mutants ofAcinetobactersp. Strain ADP1., Appl. Env. Microbiol. (May 2004), vol. 70, No. 5, pp. 2974-2983.
Subrahmanyam et al., Overproduction of a Functional Fatty Acid Biosynthesis Enzyme Blocks Fatty Acid Synthesis inEscherichia coli.; J. Bacteriology (Sep. 1998), vol. 180, No. 17, pp. 4596-4602.
Murli et al., Metabolic engineering ofEscherichia colifor improved 6-deoxyerythronolide B production. J Ind Microbiol Biotechnol (2003) 30: 500-509.
Ro et al., Production of the antimalarial drug precursor artemisinic acid in engineered yeast, Nature 2006, 440:940.
Reiling et al., Mono and Diterpene Production inEscherichia coli. Biotechnol. Bioeng. 2004, 87: 200-212.
Song, Lisheng. A Soluble Form of Phosphatase inSaccharomyces cerevisiaeCapable of Converting Farnesyl Diphosphate Into E,E-Farnesol. Appl. Biochem. Biotechnol. (2006) 128: 149-157.
Takami et al., Complete genome sequence of the alkaliphilic bacteriumBacillus haloduransand genomic sequence comparison withBacillus subtilis. Nucleic Acids Research, 2000, vol. 28, No. 21, pp. 4317-4331.
Kunst et al. The complete genome sequence of the Gram-positive bacteriumBacillus subtilis; Nature (1997), 390:249-256.
Pearson et al., Journal of Bateriology (2000) 182: 4121-4123.
Mildvan et al. Structures and mechanisms of Nudix hydrolases., Arch. Biochem. Biophysics (2005) 433: 129.
Allen et al., Phosporyl group transfer: evolution of a catalytic scaffold. Trends Biochem. Sci. (2004) 29: 495.
Rigden et al., Structures of Phosphate and Trivanadate Complexes ofBacillus stearothermophilusPhosphatase PhoE: Structural and Functional Analysis in the Cofactordependent Phosphoglycerate Mutase Superfamily. J. Mol. Biol. (2003) 325: 411.
McLennan, A.G., The Nudix hydrolase superfamily. Cellular and Mol. Life Sci. (2006), vol. 63, No. 2, pp. 123-143.
Kuznetsova et al., Genome-wide Analysis of Substrate Specificities of theEscherichia coliHaloacid Dehalogenase-like Phosphatase Family. J. Biol. Chem. (2006) vol. 281, No. 47, pp. 36149-36161.
Ward et al., Reductive biotransformations of organic compounds by cells or enzymes of yeast. Enzyme Microb. Technol., 1990, vol. 12, pp. 482-493.
Rodriguez et al., Highly Stereoselective Reagents for Beta-Keto Ester Reductions by Genetic Engineering of Baker's Yeast., J. Am. Chem. Soc. (2001), vol. 123, No. 8, pp. 1547-1555.
King et al., Biotransformation of monoterpene alcohols bySaccharomyces cerevisiae, Torulaspora delbrueckiiandKluyveromyces lactis. Yeast (2000) 16: 499-506.
Williams et al., ‘New uses for an Old Enzyme’ —The Old Yellow Enzyme family of flavoenzymes., Microbiology (2002) 148:1607-1614.
Larroy et al., Characterization of aSaccharomyces cerevisiaeNADP(H)-dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase family. Eur. J. Biochem. 269, 5738-5745 (2002).
Dickinson et al., The activity of yeast ADH I and ADH II with long-chain alcohols and diols. Chemico-Biological Interactions (2001) vol. 130-132, pp. 417-423.
International Preliminary Report on Patentability and Written Opinion of the ISA, PCT/US2008/068831; mailed Oct. 9, 2008.
International Application Published under the Patent Cooperation Treaty, WO 2009/006429A1, Jan. 8, 2009, and ISR.
Chou Howard H.
Keasling Jay D.
Chiang Robin C.
Lawrence Berkeley National Laboratory
Nashed Nashaat
The Regents of the University of California
LandOfFree
Host cells and methods for producing 3-methyl-2-buten-1-ol,... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Host cells and methods for producing 3-methyl-2-buten-1-ol,..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Host cells and methods for producing 3-methyl-2-buten-1-ol,... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2737941