Diagnostic method and products useful therein

Details Diagnostic method and products useful therein Diagnostic method and products useful therein

Filed on

2011-06-14

Date issued

2011-06-14

Examiner

Babic, Christopher M. (Department: 1637)

Patent Class

Chemistry: molecular biology and microbiology

SubClass

Measuring or testing process involving enzymes or...

SubSubClass

Involving nucleic acid

Type

Reexamination Certificate

Status

active

Patent number

07960106

Description

ABSTRACT:
A method for simultaneous detection and identification ofBacillus anthracis, Yersinia pestisandFrancisella tularensis, in a single real time PCR assay using species-specific primers and Taqman MGB probes. Also, a kit for the diagnosis of bacterial bioterrorism agents. In addition, an infection-free control plasmid to verify the result of the real time PCR analysis method.

REFERENCES:
patent: 6329156 (2001-12-01), Cirino et al.
patent: 7695941 (2010-04-01), Lin et al.
patent: 2003/0082563 (2003-05-01), Bell et al.
patent: 2004/0185438 (2004-09-01), Ecker
patent: 2004/0259226 (2004-12-01), Robey et al.
patent: WO 03/075837 (2003-09-01), None
patent: WO 2004/070001 (2004-08-01), None
patent: WO 2005/030991 (2005-04-01), None
Buck et al (“Design Strategies and Performance of Custom DNA Sequencing Primers” Biotechniques. 1999. 27(3): pp. 528-536.
Lowe et al. (Nucleic Acids Research, vol. 18, No. 7, p. 1757-1761, 1990).
Wilson et al. (“A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents” Molecular and Cellular Probes, Academic Press, London, GB, vol. 19, No. 2, Apd12005 (Apr. 2005), pp. 137-144).
Loiez et al. (“Detection of Yersinia pestis in Sputum by Real-Time PCR” Journal of Clinical Microbiology, vol. 41, No. 10, Oct. 2003, pp. 4873-4875).
Versage et al. (“Development of multitarget Real-Time TaqMan PCR assay for enhanced detection ofFrancisella tularensisin complex specimens” Journal Ofclinical Microbiology, vol. 41, No. 12, Dec. 2003, pp. 5492-5499).
GENBANK Accession Nos. M27820 (Apr. 26, 1993).
GENBANK Accession Nos. Y08861 (Feb. 16, 1997).
Charrel, R.N. et al., “Multi-Pathogens Sequence Containing Plasmids as Positive Controls for Universal Detection of Potential Agents of Bioterrorism,” BMC Micorbiology, vol. 4, pp. 1-11, 2004.
Loiez e, C. et al., “Detection of Yersinia Pestis in Sputum by Real-Time PCR,” Journal of Clinical Microbiology, vol. 41, No. 10, pp. 4873-4875 Oct. 2003.
Versage, J.L. et al., “Development of a Multitarget Real-Time TaqMan PCR Assay for Enhanced Detection ofFrancisella tularensisin Complex Specimens,” Journal of Clinical Microbiology, vol. 41, No. 12, pp. 5492-5499, Dec. 2003.
Ellerbrok, Heinz et al. “Rapid and sensitive identification of pathogenic and apathogenicBaccilus anthracisby real-time PCR”,FEMS, Letters, vol. 214, pp. 51-59, 2002.
Hanna, P. “Anthrax Pathogenesis and Host Response”,Curr. Top. Microbiol. Immunol., vol. 225, pp. 13-35, 1988.
Bastien, M. et al. “A Specific, Sensitive and Rapid (<1hr) Assay for the Detection of the Biothreat AgentFrancisella tularcusis”, Abstracts, ASM Biodefense Research Meeting, 2005.
Reif, Timothy C. et al. “Identification of Capsule-FormingBaccillus anthracisSpores with the PCR and a Novel Dual-Probe Hybridization Format”,Applied and Environmental Microbiology, vol. 60, No. 5, pp. 1622-1625, May 1994.
Dokusova, Lucia et al. “Confirmation of anthrax occurrence using real-time PCR”,Biologia, bratislava, vol. 59, No. 6, pp. 803-807, Nov. 2004.
Wilson, Wendy et al. “A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents”,Molecular and Cellular Probes, vol. 19, No. 2, pp. 137-144, Apr. 2005.
Chase, C.J. et al. “Real-Time PCR Assays Targeting a Unique Chromosomal Sequence forYersinia pestis”,Clin. Chem., Aug. 11, 2005.
Bell, Constance A. et al. “Detection ofBacillus anthracisDNA by LightCycler PCR”,Journal of Clinical Microbiology, vol. 40, No. 8, pp. 2897-2902, Aug. 2002.
Emanuel, P. A. et al. “Detection ofFrancisella tulareniswithin infected mouse tissues by using a hand-held PCR thermocycler”,J. Clin. Microbiol., vol. 41, No. 2, pp. 689-693, Feb. 2003.
Mandira, Varma-Basil. “Molecular Beacons fo rMultiplex Detection of Four Bacterial Bioterrorism Agents”,Clinical Chemistry, vol. 50, No. 6, pp. 1060-1063, 2004.
Hurtle et al., “Detection of theBacillus anthracis gyrAGene by Using a Minor Groove Binder Probe”, Journal of Clinical Microbiology, vol. 42, No. 1, pp. 179-185, 2004.
Jun. 11, 2010 European Office Action issued in European Patent Application No. 06 125 578.2.

Affiliated with

Also associated with

Rating

Diagnostic method and products useful therein does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Diagnostic method and products useful therein, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Diagnostic method and products useful therein will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2733962