Individual-cell electroporation using area-focused electric...

Details Individual-cell electroporation using area-focused electric... Individual-cell electroporation using area-focused electric...

2011-08-30

2011-08-30

Bowers, Nathan A (Department: 1775)

Chemistry: molecular biology and microbiology

Apparatus

Mutation or genetic engineering apparatus

C435S173100, C435S173600, C435S460000, C435S461000, C204S403010

Reexamination Certificate

active

08008063

ABSTRACT:
Electroporation is performed on a population of cells, liposomes, vesicles, or other membrane-encased structures with uniform results regardless of size variations within the population, by drawing the membrane-encased structures into micron-sized openings that contain paired electrodes. An electric potential is then imposed between the paired electrodes to permeabilize only that portion of each cell that extends into the openings and resides within the electric field focused in the area between the electrodes.

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patent: 2008/0138876 (2008-06-01), Ragsdale

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