Tube gel electrophoresis device

Details Tube gel electrophoresis device Tube gel electrophoresis device

1976-11-19

1977-09-13

Prescott, Arthur C.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204180G, G01N 2726

Patent

active

040480496

ABSTRACT:
A device for performing electrophoretic separation of samples in a tube shaped gel. An outer container has an open upper end and side walls joined by a bottom closure. An upper buffer chamber assembly is received in and supported by the open upper end of the outer container. The upper buffer assembly defines an upper buffer chamber therein having a bottom wall spaced from the bottom closure of the outer container and thereby together with the outer container sidewalls forming a lower buffer chamber. A removable cooling core is disposed in the lower buffer chamber and has a cooling passage therethrough having open ends in communication with the lower buffer chamber. A plurality of tubes for holding electrophoresis separation gels are provided which extend through the bottom wall of the upper buffer chamber, thereby having one end in communication with each of the upper and lower buffer chambers. An electrode is provided in each of the buffer chambers so that when buffer solution is contained in each buffer chamber and voltage is impressed across the electrodes, the sample is electrophoretically separated in the separation gel in the tubes and heat energy generated within the separation gels is passed to the buffer solution in the lower buffer chamber. Circulation of the buffer solution through the cooling passages in the cooling core in turn transfers the heat energy to a coolant passing therethrough.

REFERENCES:
patent: 3699033 (1972-10-01), Zeineh
patent: 3773648 (1973-11-01), Welzen et al.
patent: 3867271 (1975-02-01), Hoefer
patent: 3950546 (1976-09-01), Caccavo
patent: 3956099 (1976-05-01), Israel et al.

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