Antisense oligonucleotide modulation of multidrug resistance-ass

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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435 6, 435 911, 4351713, 435 325, 536 231, 536 243, 536 245, C12N 1585, A61K 4800, C12Q 168, C07H 2104

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active

058011544

ABSTRACT:
Compositions and methods are provided for the treatment and diagnosis of diseases or conditions amenable to treatment through modulation of the synthesis or metabolism of multidrug resistance-associated protein (MRP). In accordance with referred embodiments, oligonucleotides are provided which are specifically hybridizable with nucleic acids encoding MRP. In a preferred embodiment, the oligonucleotide has at least one 2'-methoxyethoxy modification. Methods of preventing the development of multidrug resistance and of improving the efficacy of chemotherapy are also disclosed.

REFERENCES:
patent: 5034506 (1991-07-01), Summerton et al.
patent: 5138045 (1992-08-01), Cook et al.
patent: 5218105 (1993-06-01), Cook et al.
patent: 5459255 (1995-10-01), Cook et al.
Canitrot et al., "MRP-associated multidrug resistance its reversal by 2'-modified antisense oligonucleotides", Anti-Cancer Drugs 1996, 7(suppl. 3), 93-99.
Cole et al., "Rapid chemosensitivity testing of human long tumor cells using the MTT assay", Cancer Chemother. Pharmacol. 1986, 17, 259-263.
Cole et al., "Overexpression of a Transporter Gene in Multidrug-Resistant Human Lung Cancer Cell Line", Science 1992, 258, 1650-1654.
Cole et al., "Multidrug Resistance-Associated Protein: Sequence Correction", Science 1993, 260, 879.
Crooke et al., "Pharmacokinetic Properties of Several Novel Oligonucleotide Analogs in mice", J. Pharmacol. Exp. Ther. 1996, 277, 923-937.
De Mesmaeker et al., "Antisense Oligonucleotides", Acc. Chem. Res. 1995, 28, 366-374.
Gao et al., "Reconstitution of ATP-dependent Leukotriene C.sup.4 Tranport by Co-expression of Both Half-molecules of Human Multidrug Resistance Protein in Insect Cells", J. Biol. Chem. 1996, 271, 27782-27787.
Gebeyehu et al., "Novel biotinylated nucleotide--analogs for labeling and colorimetric detection of DNA", Nucl. Acids Res. 1987, 15,4513-4534.
Henderson, W.R., Jr., "The Role of Leukotrienes in Inflammation", Ann. Int. Med. 1994, 121, 684-697.
Kabanov et al., "A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus-specific proteins in MDCK cells", FEBS Lett. 1990, 259, 327-330.
Kornberg, A., "Uncommon Nucleotides", DNA Replication, W.H. Freeman & Co., San Francisco, 1980, pp. 75-77.
Letsinger et al., "Cholesteryl-conjugated oligonucleotides: Synthesis, properties, and activity as inhibitors of replication of human immunodeficiency virus in cell culture", Proc. Natl. Acad. Sci. USA 1989, 86, 6553-6556.
Manoharan et al., "Cholic Acid-Oligonucleotides Conjugates for Antisense Applications", Bioorg. Med. Chem. Let. 1994, 4, 1053-1060.
Manoharan et al., "Chemical Modifications to Improve Uptake and Bioavailability of Antisense Oligonucleotides", Ann. N.Y. Acad. Sci. 1992, 660, 306-309.
Manoharan et al., "Introduction of a Lipophilic Thioether Tether in the Minor Groove of Nucleic Acids for Antisense Applications", Bioorg. Med. Chem. Let. 1993, 3, 2765-2770.
Manoharan et al., "Oligonucleotides Conjugates: Alteration of the Pharmacokinetic Properties of Antisense Agents", Nucleosides & Nucleotides 1995, 14, 969-973.
Manoharan et al., "Lipidic Nucleic Acids", Tetrahedron Lett. 1995, 36, 3651-3654.
1993 Catalog of Products for DNA Research, Glen Research, Sterling, VA, p. 21.
Martin, P., "Ein neuer Zugang zu 2'-O-Alkylribonucleosiden und Eigenschaften deren Oligonucleotides", Helv. Chim. Acta 1995, 78,486-504.
Mirski et al., "Multidrug Resistance in Human Small Cell Lung Cancer Cell Line Selected in Adriamycin", Cancer Res. 1987, 47, 2594-2598.
Mirski et al., "Antigens Associated with Multidrug Resistance in H69AR, a Small Cell Lung Cancer Cell Line", Cancer Res. 1989, 49, 5719-5724.
Mishra et al., "Improved leishmanicidal effect of phosphorothioate antisense oligonucleotides by LDL-mediated delivery", Biochim. Biophys. Acta 1995, 1264, 229-237.
Neilsen et al. "Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide", Science 1991, 254, 1497-1500.
Oberhauser et al., "Effective incorporate of 2'-O-methyl-oligoribonucleotides into liposomes and enhanced cell associated through modification with thiocholesterol", Nucl. Acids. Res. 1992, 20, 533-538.
Sambrook et al., Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, 1989, vol. 2, pp. 10.59-10.61.
Sambrook et al., "Molecular Cloning. A Laboratory Manual," Cold Spring Harbor Laboratory Press, 1989, vol. 2, pp. 11.31-11.32.
Saison-Behmoaras et al., "Short modified antisense oligonucleotides directed against Ha-ras point mutation induce selective cleavage of the mRNA and inhibit T24 cells proliferation", EMBO J. 1991, 10, 1111-1118.
Sanghvi, Y.S., in Antisense Research and Applications, Crooke and Lebleu, eds., CRC Press, Boca Raton, 1993, pp. 273-288.
Shea et al., "Synthesis, hybridization properties and antiviral activity of lipid-oligodeoxynucleotide conjugates", Nucl. Acids Res. 1990, 18, 3777-3783.
Slovak et al., "Localization of Novel Multidrug Resistance-associated Gene in the HT1080/DR4 and H69AR Human Tumor Cell Lines", Cancer Res. 1993, 53, 3221-3225.
Svinarchuk et al., "Inhibition of HIV proliferation in MT-4 cells by antisense oligonucleotide conjugated to lipophilic groups", Biochimie 1993, 75, 49-54.
Thierry et al., "Overcoming Multidrug Resistance in Human Tumor Cells Using Free and Liposomally Encapsulated Antisense Oilgodeoxynucleotides", Biochem. Biophys. Res. Comm. 1993, 190, 652-960.
Vasanthakumar, G. and N.K. Ahmed, "Modulation of Drug Resistance in a Daunorubicin Rsistant Subline with Oligonucleoside Methylphosphonates", Cancer Commun. 1989, 1, 225-232.

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