Reverse cumate repressor mutant

Details Reverse cumate repressor mutant Reverse cumate repressor mutant

2011-05-03

2011-05-03

Steadman, David J (Department: 1656)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Reexamination Certificate

active

07935788

ABSTRACT:
Recently, the development of inducible expression systems has involved exploitation of the p-cym operon fromPseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of a CymR variant with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variant, fusion proteins incorporating such a variant, and its use in the control and expression of polynucleotides. The CymR variant comprises a 142Glu to 142Gly single point mutation of wild type CymR.

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