Methods for direct fluorescent antibody virus detection in...

Details Methods for direct fluorescent antibody virus detection in... Methods for direct fluorescent antibody virus detection in...

2011-08-23

2011-08-23

Mosher, Mary E (Department: 1648)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

C435S007100

Reexamination Certificate

active

08003314

ABSTRACT:
The present invention describes a liquid direct fluorescence antibody assay that is rapid and sensitive to detect respiratory virus in infected cells. The assay includes centrifugation of the specimen, incubation of sample and reagents in solution, and detection of the absence or presence of respiratory virus. Sapogenin is used as a detergent to permeabilize the cells for entry of the monoclonal antibodies to react with intracellular antigens. The cells are stained with fluorescently labeled monoclonal antibodies against the viral antigens along with a background stain and a fluorescent nuclear stain. This counter staining decreases background and allows co-localization of antigen and nuclear structures for enhanced detection.

REFERENCES:
patent: 6225046 (2001-05-01), Vesey et al.
Iannelli et al (Journal of Virological Methods 69:137-145, 1997).
Yan et al (Journal of Immunological Methods 284:27-38, 2004).
Boonpucknavig et al (American Journal of Pathology 85:37-48, 1976).
Martins (Clinical and Diagnostic Laboratory Immunology 9:41-45, 2002).
Alexa Fluor® data sheet.[online], [retreived on Jul. 7, 2010]. Retrieved from the internet <URL: http://www.invitrogen.com/site/us/en/home/brands/Molecular-Probes/Key-Molecular-Probes-Products/alexa-fluor/Alexa-Fluor-488-Secondary-Antibodies.html>.
Rey Nores et al (Journal of General Virology 76:2779-2791, 1995).
Chan et al (Clinical and Diagnostic Laboratory Immunology 8:909-912, 2001).
Schmid et al (Journal of Immunological Methods 247:175-186, 2001).

Affiliated with

Also associated with

Rating

Methods for direct fluorescent antibody virus detection in... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for direct fluorescent antibody virus detection in..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for direct fluorescent antibody virus detection in... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2696576