Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Patent
1997-09-29
1998-09-01
Ketter, James
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
436 66, G01N 3372
Patent
active
058010530
ABSTRACT:
Improved HPLC methods for the identification and characterization of hemoglobin variants in blood samples are disclosed. These methods are performed manually or with the aid of a computer. In preferred embodiments, a blood sample is injected into an HPLC unit and hemoglobin species from the blood sample are separated using high column temperatures and high eluant flow rates. Blood samples are initially screened using a low-resolution HPLC method to determine if an eluted hemoglobin species has a characteristic indicative of a hemoglobin variant, i.e., if a chromatograph peak of the eluted species has one of a number of parameter values falling within a predetermined range of values. If the initial screening indicates that a blood sample may have a hemoglobin variant, the blood sample is subsequently reanalyzed using a high-resolution HPLC method to determine if an eluted hemoglobin species corresponds to a known hemoglobin variant by comparing the retention time of an eluted species to the retention times of known hemoglobin variants. Alternatively, both the low-resolution and high-resolution HPLC methods can be used independently of one another. Thus, the methods of the invention are useful in putatively identifying abnormal hemoglobins.
REFERENCES:
patent: 4269605 (1981-05-01), Dean et al.
patent: 4371374 (1983-02-01), Cerami et al.
patent: 4409335 (1983-10-01), Hanamoto et al.
patent: 4507390 (1985-03-01), Hotiuchi et al.
patent: 4767529 (1988-08-01), Boos et al.
patent: 4802981 (1989-02-01), Kenney et al.
patent: 4861728 (1989-08-01), Wagner
patent: 5137833 (1992-08-01), Russel
Reid et al.; Applications for group specific ligands in fast, high performance affinity chromatography; Biochromatography, vol. 3,No. 5 (1988).
Middle et al.; Separation of glycosylated hemoglobins using immobilized phenylboronic acid; Biochem. J., 209:771-779 (1985).
Gould et al.; Measurement of glycosylated hemoglobins using an affinity chromatography method; Clinica Chimica Acta; 125:41-48 (1982).
Mallia et al.; Preparation and use of a boronic acity affinity support for separation and quantitation of glycosylated hemoglobins; Analytical Letters; 14(B8):649-661 (1981).
Dean et al.; Applications of immobilised Boronic acids; Affinity Chromatography and Biological Recognition; 433-443 (1983).
Bannon et al.; Glycated hemoglobin in uremic patients as measured by affinity an ion-exchange chromatography, Clinical Chemistry; 30(3):485-486 (1984).
Little et al.; Glycosylated hemoglobin measured by affinity chromatography: Microsample collection and room-temperature storage; Clin. Chem., 29(6):1080-1082 (1983).
Willey et al.; Glycosylated haemoglobin and plasma glycoprotein assays by affinity chromatography; Diabetologia, 27:56-58 (1984).
Papadea eet al.; The effect of storage conditions on ion exchange and affinity chromatographic assays for glycated hemoglobin; Clinical Biochemistry, 17:296-301 (1984).
Talwar et al.; Determination of glycosylated adult and foetal haemoglobins by affinity chromatography: Clinical Chimica Acta., 128:61-67 (1983).
John et al.; Affinity chromatography method for the measurement of glycosylated haemoglobin: comparison with two methods in routine use; Clinica Chimica Acta., 136:257-262 (1984).
Ou et al.; Rapid Analysis of Hemoglobin Variants by Cation-Exchange HPLC Clin. Chem.; 39/5, 820-824 (1993).
Turpeinen et al.; Two .alpha.-Chain Hemoglobin Variants, Hb Broussais and Hb Bemenelum, Characterized by Cation-Exchange HPLC, Isoelectric Focusing and Peptide Sequencing; Clin. Chem. 41/4, 532-536 (1995).
Huisman, Separation of Hemoglobins and Hemoglobin Chains by High-Performance Liquid Chromatography; J. Chromatog. 418 (1987) 277-304.
Turpeinen; Liquid Chromatographic Determination of Hemoglobin A.sub.2 ; Clin. Chem. 32/6, 999-1002 (1986).
Huisman et al.; Microchromatography of hemoglobins. III. A simplified procedure of the determination of hemoglobin A.sub.2 ; J. Lab. Clin. Med. (1975); vol. 86, No. 4, pp. 700-703.
Bisse et al.; High-Perofrmance Liquid Chromatographic Separation of Human Haemoglobins; J. Chromatog, 434 (1988) 95-110.
Roger s et al.; High-Performance Liquid Chromatography in the Diagnosis of Hemoglobinopathies and Thalassemias; A.J.C.P. (1985), vol. 84, No. 5, pp. 671-674.
Pembrey et al.; Reliale routine estimate of small amounts of foetal haemoglobin by alkali denaturation; J. Clin. Path., vol. 25 (1972) 738-740.
Wessels et al.; Liquid Chromatography Used in Diagnosis of a Rare Hemoglobin Combination: Hemoglobin S/Lepore; CLin. Chem. 32/5, 903-906 (1986).
Wilson et al.; A New High-Performance Liquid Chromatographic Procedure for the Separation and Quantitation of Various Hemoglobin Variants in Adults and Newborn Babies; Wilson et al.; J. Lab. Clin. Med. 102: 174, 1983.
Ou et al.; High-Performance Liquid Chromatography of Human Hemoglobins on a New Cation Exchanger; J. Chromatog. 266 (1983) 197-205.
Roa et al.; Reference Ranges for Hemoglobin Variants by HPLC in African Americans; Ann. Clin & Lab. Sci., vol. 25, No. 3 (1995 228-235.
Abstract No. 222; A cation-exchange high performance liquid chromatographic assay for hemoglobin A1c and hemoglobin screening; Clin. Chem., vol. 31, No. 6, (1985).
Klenk et al. Determination of glycosylated hemoglobin by affinity chromatography: comparison with colorimetric and ion-exchange methods, and effects of common interferences; Clin. Chem., 28(10) (1982).
Little et al.; Effects of whole blood storage on results for glycosylated hemoglobin as measured by ion-exchange chromatography, affinity chromatography and colorimery; Clin. Chem., 29(6):1113-1115 (1983).
Peterson et al.; A comparative evaluation of glycosylated haemoglobin assays: feasibility of references and standards; Diabetologia,26:214-217 (1984).
Herold et al.; Measurement of glycosylated hemoglobins using boronate affinity chromatography; Ann. Clin & Lab. Sci., 13(6):482-488 (1983).
Gould et al.; A sensitive method for the measurement of glycosylated plasma proteins using affinity chromatography; Am. Clin. Biochem., 21:16-21 (1984).
Mullins et al.; Sensitivity of isoelectric focusing, ion exchange and affinity chromatography to labile glycated hemoglobin; Clin. Chem., 32(8):1460-1463 (1986).
Dean; Measurement of glycosylated haemoglobins using affinity chromatography; Diabetologia, 21:579-580 (1981).
Hall et al.; Measurement of glycosylated haemoglobins abd glycosylated plasma proteins in maternal and cord bloodusing an affinitychromatography method; Diabetologia, 25:477-481 (1983).
Bruns et al.; Specific affinity-chromatographic measurement of glycated hemoglobins in uremic patients; Clin. Chem., 30(4):569-571 (1984).
Abraham et al.; application ofaffinity chromatography for separation and quantitation of glycosylated hemoglobins; J. Lab. Clin. Med., 187-197 (1983).
Fairbanks et al.; Measurement of glycosylated hemoglobin byaffinity chromatography; Mayo Clin. Proc., 58:770-773 (1983).
McLennan et al.; The measurement of glycosylated hemoglobin in man and animals by aminophenylboronic acid affinity chromatography; Diabetes, 31:701-705 (1982).
Hall et al.; Glycosylated hemoglobins and glycosylated plasma proteins in the diagnosis of diabetes mellitus and impaired glucose tolerance; Diabetes Care, 7(2):147-150 (1984).
Fluckiger et al.; Measurement of nonenzymatic protein glycosylation; Methods in Enzymology, 106:77-87 (1984).
Yatscoff et al.; Evaluation of an affinity chromatographic procedure for the determination of glycosylated hemoglobin (HbA1); source unknown; pp. 291-295.
Garlick et al.; Characterization of glycosylated hemoglobins; J. Clin. Invest., 71:1062-1072 (1983).
Glycosylated Albumin; Endocrine Sciences.
Noffsinger Jimmie K.
Ou Ching-Nan
Ketter James
Primus Corporation
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