Method of determining the mutational load of a gene library...

Details Method of determining the mutational load of a gene library... Method of determining the mutational load of a gene library...

2011-07-19

2011-07-19

Skowronek, Karlheinz R (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C536S025300

Reexamination Certificate

active

07983849

ABSTRACT:
The invention relates to methods of determining the mutational load of a gene bank obtained by random mutagenesis of a gene of interest by preparing a chart linking the mutational load of a gene bank obtained by random mutagenesis of a model gene with the fraction of mutated model genes observed in the bank; performing, in parallel, random mutagenesis of the model gene used for preparing the chart and the gene of interest to obtain the corresponding mutated gene banks; determining the mutational load of the gene bank obtained using the model gene on the basis of the chart plotted; and applying a correction factor to the mutational load of the mutated model gene bank to determine the mutational load in the gene bank of the mutated genes of interest.

REFERENCES:
patent: 2001/0037454 (2001-11-01), Botti et al.
patent: 2003/0152944 (2003-08-01), Hogrefe et al.
Patrick et al. (Protein Engineering, vol. 16, No. 6, p. 451-457, Jun. 2003).
Vu et al. (Nucleic acids research, vol. 28, No. 7, e18, pp. 9, 2000).
Cirino et al. (From: Methods in Molecular Biology, vol. 231: Directed Evolution Library Creation: Methods and Protocols Edited by: F. H. Arnold and G. Georgiou O, Humana Press Inc., Totowa, NJ, p. 3-9, 2003).
Wilson et al.( Current Protocols in Molecular Biology (2000) 8.3.1-8.3.9 Copyright © 2000 by John Wiley & Sons, Inc.).

Affiliated with

Also associated with

Rating

Method of determining the mutational load of a gene library... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of determining the mutational load of a gene library..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of determining the mutational load of a gene library... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2690583