Materials and methods for the efficient production of...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S183000, C435S006120, C435S252330, C435S190000, C435S189000, C435S193000, C435S195000, C435S252300, C536S023200, C536S023100

Reexamination Certificate

active

07977075

ABSTRACT:
The subject invention provides materials and methods wherein unique and advantageous combinations of gene mutations are used to direct carbon flow from sugars to a single product. The techniques of the subject invention can be used to obtain products from native pathways as well as from recombinant pathways. In preferred embodiments, the subject invention provides new materials and methods for the efficient production of acetate and pyruvic acid.

REFERENCES:
patent: 6013494 (2000-01-01), Nakamura et al.
patent: 6159738 (2000-12-01), Donnelly et al.
patent: 7098009 (2006-08-01), Shanmugam et al.
patent: 2007/0037265 (2007-02-01), Zhou et al.
patent: 2007/0072280 (2007-03-01), Cirino et al.
patent: 0 162 545 (1985-11-01), None
patent: WO 87/03006 (1987-05-01), None
patent: WO 98/10089 (1998-03-01), None
patent: WO 2008/115958 (2008-09-01), None
patent: WO 2008/119009 (2008-10-01), None
Stewart et al. Kirkpatrick et al. (Biotechnology and Genetic Engineering Reviews. Vo. 14, Apr. 1997, pp. 67-143.
Causey, T. B. et al. “EngineeringEscherichia colifor efficient conversion of glucose to pyruvate,”PNAS, Feb. 24, 2004, vol. 101, No. 8, pp. 2235-2240, XP-002519088.
Girbal, L. et al. “Regulation ofClostridium acetobutylicumMetabolism as Revealed by Mixed-Substrate Steady-State Continuous Cultures: Role of NADH/NAD Ratio and ATP Pool”Journal of Bacteriology, Nov. 1994, vol. 176, No. 21, pp. 6433-6438, XP-002519087.
Aristidou, A. A. et al. “Metabolic engineering ofEscherichia colito enhance recombinant protein production through acetate reduction,”Biotechnol. Prog. (1995), vol. 11, pp. 475-478.
Beck, B.J. et al. “Iterative chain elongation by a pikromycin monomodular polyketide synthase”J. Am. Chem. Soc. (2003), vol. 125, pp. 4682-4683.
Berraud, C. “Production of highly concentrated vinegar in fed-batch culture,”BiotechnoL lett. (2000), vol. 22, pp. 451-454.
Causey, T.B. et al. “EngineeringEscherichia coliW3110 for the conversion of sugar to redox-neutral and oxidized products: Homoacetate production,”Proc. Natl. Acad. Sci., USA(2003), vol. 100, pp. 825-832.
Chang, Y. et al. “Expression ofEscherichia colipyruvate oxidase (PoxB) depends on the sigma factor encoded by therpoS(katF) gene,”Mol. Microbiol. (1994), vol. 11, pp. 1019-1028.
Chao, Y. and Liao, J.C. “Metabolic responses to substrate futile cycling inEscherichia coli,” J. Biol. Chem. (1994), vol. 269, pp. 5122-5126.
Contiero, J. et al. “Effects of mutations in acetate metabolism on high-cell-density growth ofEscherichia coli,” J. Ind. Microbiol. (2000), vol. 24, pp. 421-430.
Datsenko, K.A. etal. “One-step inactivation of chromosomal genes inEscherichia coliK-12 using PCR products,”Proc. Natl. Acad. Sci., USA(2000), vol. 97, pp. 6640-6645.
Dien, B.S. et al. “RecombinantEscherichia coliengineered for the production of L-lactic acid from hexose and pentose sugars”J. Ind. Microbiol. Biotechnol. (2001), vol. 27, pp. 259-264.
Farmer, W.R. et al. “Reduction of aerobic acetate production byEscherichia coli,” Appl. Environ. Microbiol. (1997), vol. 63, pp. 3205-3210.
Freer, S.N. “Acetic acid production byDekkera/Brettanomycesyeasts”World J. Microbiol. Biotechnol. (2002), vol. 18, pp. 271-275.
Hofmeyer et al. “Regulateing the cellular economy of supply and demand,”FEBS Lett. (2000), vol. 476, pp. 47-51.
Ingram, L. O. et al. “Enteric Bacterial catalyst for fuel ethanol production”Biothechnol. Prog. (1999), vol. 15, pp. 855-866.
Kirkpatrick, C. et al. “Acetate and formate stress: Opposite responses in the proteomes ofEscherichia coli,” J. Bacteriol. (2001), vol. 183, pp. 6466-6477.
Koebmann, B.J. et al. “The glycolytic flux inEscherichia coliis controlled by the demand for ATP,”J. Bacteriol. (2002), vol. 184, pp. 3909-3916.
Lasko, D.R. et al. “Bacterial response to acetate challenge: a comparison of tolerance among species,”Appl. Microbiol. Biotechnol. (2000), vol. 54, pp. 243-247.
Li, Y. et al. “Biotechnological production of pyruvic acid,”Appl. Microbiol. Biotechnol. (2001), vol. 57, pp. 451-459.
Li, Y. et al. “Efficient pyruvate production by a multi-vitamin auxotroph ofTorulopsis glabrata: key role and optimization of vitamin levels,”Appl. Microbiol. Biotechnol. (2001), vol. 55, pp. 680-688.
Ljungdahl, L.G. “The autotrophic pathway of acetate synthesis in acetogenic bacteria,”Ann. Rev. Microbiol. (1986), vol. 40, pp. 415-450.
Luli, G.W. and R. Strohl “Comparison of growth, acetate production and acetate inhibition ofEscherichia colistrains in batch and fed-batch fermentations,”Appl. Environ. Microbiol. (1990), vol. 56, pp. 1004-1011.
Martinez-Morales, F. et al. “Chromosomal integration of heterologous DNA inEscherichia coliwith precise removal of markers and replicons during construction,”J. Bacteriol. (1999), vol. 181, pp. 7143-7148.
Niu, W. et al. “Benzene-free synthesis of adipic acid”Biotechnol. Prog. (2002), vol. 18, pp. 201-211.
Patnaik, R. et al. “Stimulation of glucose catabolism inEscherichia coliby a potential futile cycle,”J. Bacteriol. (1992), vol. 174, pp. 7527-7532.
Posfai, G. et al. “Versatile insertion plasmids for targeted genome manipulation in bacteria: Isolation, deletion, and rescue of the pathogenicity island LEE of theEscherichia coliO157:H7 genome,”J. Bacteriol. (1997), vol. 179, pp. 4426-4428.
Tomar, A. et al. “The effect of acetate pathway mutations on the production of pyruvate inEscherichia coli,” Appl. Microbiol. Biotechnol. (2003), vol. 62, pp. 76-82.
Tong, I. et al. “1,3-propanediol production byEscherichia coliexpressing genes from the Klebsiella-pneumoniae-DHA regulon”Appl. Environ. Microbiol. (1991), vol. 57, pp. 3541-3546.
Underwood, S. et al. “Genetic changes to optimize carbon partitioning between ethanol and biosynthesis in ethanologenicEscherichia coli” Appl. Environ. Microbiol. (2002), vol. 68, pp. 6263-6272.
Underwood, S. et al. “Flux through citrate synthase limits the growth of ethanologenicEscherichia coliKO11 during xylose fermentation,”Appl. Environ. Microbiol. (2002), vol. 68, pp. 1071-1081.
Varma, A et al. “Stoichionetric interpretation ofEscherichia coliglucose catagolism under various oxygenation rates,”Appli. Environ. Microbiol. (1993), vol. 59, pp. 2465-2473.
Wang et al. “Directed evolution of metabolically engineeredEscherichia colifor carotenoid production”Biotechnol. Prog. (2000), vol. 16, pp. 922-926.
Yokota, A. et al. Pyruvic acid production by an F1-ATPase-defective mutant ofEscherichia coliW1485lip2: Biosci. Biotech. Biochem. (1994), vol. 58, pp. 2164-2167.
Zhou, S. et al. “Production of optically pure D-lactic acid in mineral salts medium by metabolically engineeredEscherichia coliW3110”Appl. Environ. Microbiol. (2003), vol. 69, pp. 399-407.
Klegg Database, Pyruvate Metabolism, TCA cycle andE. coliacetate kinase A, fumarate reductase, pyruvate formate lyase, 2-ketoglutarate dehydrogenase, D-lactate dehydrogenase, pyruvate dehydrogenase, ATP synthease and alcohol/aldehyde dehydrogenase.
Cover page of J. Bacteriol. 2001, November, pp. 6466-6477, vol. 183, No. 21.
Kirkpatrick, C. et al. “Acetate and Formate Stress: Opposite Responses in the Proteome ofEscherichia coli” Journal of Bacteriology, pp. 6466-6477, vol. 183, No. 21, 2001.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Materials and methods for the efficient production of... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Materials and methods for the efficient production of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Materials and methods for the efficient production of... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2688292

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.