Method for preparing agglutinatable, fixed platelet fragments

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...

Reexamination Certificate

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C435S013000, C436S519000

Reexamination Certificate

active

07981597

ABSTRACT:
The present invention relates to a method for preparing agglutinatable platelet fragments, in which native platelets are treated with ultrasound and a fixative. The platelet fragments are suitable for use in diagnostic assay methods which include an agglutination reaction, such as, for example, in a method for determining VWF activity.

REFERENCES:
patent: 5185160 (1993-02-01), Chao
patent: 1134584 (2001-09-01), None
patent: WO 01/02853 (2001-01-01), None
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Authi, K.S., “Preparation of Highly Purified Human Platelet Plasma and Intracellular Membranes Using High Voltage Free Flow Electrophoresis and Methods to Study Ca2+Regulation,”Platelets—A Practical Approach, Chapter 5, pp. 91-109, Oxford University Press, Editors: S.P. Watson and K.S. Authi (1996).
Federici, A.B., et al., “A Sensitive Ristocetin Co-Factor Activity Assay with Recombinant Glycoprotein lbα for the Diagnosis of Patients with Low von Willebrand Factor Levels,” Disorders of Hemostasis, 89(1):77-85 (2004).
Kitaguchi, T., et al., “Characterization of Liposomes Carrying von Willebrand Factor-Binding Domain of Platelet Glycoprotein lbα: A Potential Substitute for Platelet Transfusion,” Biochemical and Biophysical Research Communications, 261:784-789 (1999).
Lee, D.H., et al., “Platelet Substitutes and Novel Methods of Platelet Preservation,”Platelets, Chapter 60, pp. 915-924, Academic Press, Elsevier Science, Editor: A.D. Michelson (2002).
Abstracts P257-260 from Annals of Hematology, 76(suppl. 1):A92 (1998).

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