Linear polyethylenimine-sterol conjugates for gene delivery

Details Linear polyethylenimine-sterol conjugates for gene delivery Linear polyethylenimine-sterol conjugates for gene delivery

2011-01-04

2011-01-04

Badio, Barbara P (Department: 1628)

Organic compounds -- part of the class 532-570 series

Organic compounds

Cyclopentanohydrophenanthrene ring system containing

Reexamination Certificate

active

07863470

ABSTRACT:
Linear polyethylenimine was modified with sterols, such as cholesterol, in three different geometries: linear shaped (L), T-shaped (T), and a combined linear- and T-shaped (LT), to result in linear polyethylenimine-sterol conjugates. These conjugates were mixed with nucleic acids to form complexes for delivery of the nucleic acids into cells. Mammalian cells transfected with these complexes showed protein expression levels higher than linear polyethylenimine alone, and twice that of branched polyethylenimine, but without any significant loss in cell viability. Methods of making these compositions and methods of using them for gene delivery are also described.

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Lee, M., Rentz, J., Han, S.O., Bull, D.A., & Kim, S.W., Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium, 10 Gene Ther. 585-593 (2003).

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