Method for production of recombinant human FSH

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S397000, C435S398000, C530S412000

Reexamination Certificate

active

07939296

ABSTRACT:
Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.

REFERENCES:
patent: 4923805 (1990-05-01), Reddy et al.
patent: 5990288 (1999-11-01), Musick et al.
patent: 7517242 (2009-04-01), Brodersen et al.
patent: 7741455 (2010-06-01), Valax et al.
patent: 7024584 (1995-03-01), None
patent: 2523843 (1996-08-01), None
patent: 2559196 (1996-12-01), None
patent: 2001323000 (2001-11-01), None
patent: 2001323000 (2001-11-01), None
patent: 2006109696 (2006-04-01), None
patent: 8604589 (1986-08-01), None
patent: 8810270 (1988-12-01), None
patent: 2005063811 (2005-07-01), None
patent: 2006051070 (2006-05-01), None
patent: 2007065918 (2007-06-01), None
Fiddes, J.C. and H.M. Goodman. “The cDNA for the Beta-subunit of human chorionic gonadotropin suggests evolution of a gene by readthrough into the 3′-untranslated region.” (Nature), Aug. 14, 1980, 684-687, 286.
Hakola, K., et al. “Recombinant rat follicle-stimulating hormone; production by Chinese hamster ovary cells, purification and functional characterization.” Molecular and Cellular Endocrinology, vol. 127, p. 59-69, (1997).
Hjorth, R., et al. “Chromatography for Downstream Processing of Therapeutic Proteins—Current Status and Future Challenges.” AIChE Annu. Meet. Proc. Abst., (2006).
Lynch, S., et al. “The extraction and purification of human pituitary follicle-stimulating hormone and luteinizing hormone.” Acta Endocrinologica, vol. 288, p. 12-19, (1998).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for production of recombinant human FSH does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for production of recombinant human FSH, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for production of recombinant human FSH will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2673064

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.