Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2011-06-21
2011-06-21
Riley, Jezia (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023100, C536S024330, C536S026600, C435S006120, C435S091100, C435S091200, C422S067000
Reexamination Certificate
active
07964716
ABSTRACT:
The present invention is directed to a self-quenching primer comprising; a fluorophore that can be quenched by guanine; an oligonucleotide sequence that forms a hairpin; an oligonucleotide that is a target specific sequence and; use in amplification reactions, particularly in polymerase chain reactions, during which the fluorophore is released thereby emitting fluorescence.
REFERENCES:
patent: 98002449 (1998-01-01), None
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Nuovo, G.J., et al. (1999), “In Situ Amplification Using Universal Energy Transfer-Labeled Primers,” the Journal of Histochemistry & Cytochemistry, 47(3):273-279.
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Heinlein, T. et al., “Photoinduced Electron Transfer Between Fluorescent Dyes and Guanosine Residues in DNA-Hairpins,” Journal of Physical Chemistry B 20030807 American Chemical Society US, Aug. 2003, vol. 107, No. 31, pp. 7957-7964.
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Tani, H. et al., “Quantification of Genetically Modified Soybean by Quenching Probe Polymerase Chain Reaction,” Journal of Agricultural and Food Chemistry, American Chemical Society, Washington, US, Apr. 2005, vol. 53, No. 7, pp. 2535-2540.
Communication dated Apr. 17, 2009, Supplementary European Search Report, Applicant: Temasek Life Sciences Laboratory Limited, Application No. 06824649.5-2402 / 1960542 PCT/SG2006000378, 7 pages.
Hong Yan
Liu Xiao Kun
Riley Jezia
Rothwell, Figg Ernst & Manbeck, PC
Temasek Life Sciences Laboratory Limited
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