Compositions and methods for producing gamma-carboxylated...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S004000, C435S006120, C435S069100, C435S071100, C435S320100, C435S189000, C435S183000, C435S195000, C536S023200, C530S350000

Reexamination Certificate

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07989193

ABSTRACT:
The present invention relates a host cell comprising an expression vector comprising a nucleic acid molecule encoding a protein requiring gamma-carboxylation and associated expression control sequences and a nucleic acid molecule encoding a vitamin K epoxido reductase and associated expression control sequences and a nucleic acid molecule encoding a γ-glutamyl carboxylase and associated control sequences. The invention further relates to a method of producing a protein requiring gamma-carboxylation in high yields.

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