Nitrile hydratases from metagenome libraries

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C930S240000

Reexamination Certificate

active

07872100

ABSTRACT:
The present invention is concerned with the preparation of novel nitrile hydratases. These latter are preferably obtained from nonculturable organisms by means of a PCR-based screening, in metagenome DNA libraries, using special degenerate primers.

REFERENCES:
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patent: 02/053774 (2002-07-01), None
patent: 02/095053 (2002-11-01), None
GenBank Accession No. U89363.1 (Jan. 28, 2000).
GenBank Accession No. X64360 (Feb. 26, 1993).
Blast alignment of the alpha-subunit encoded by GenBank Accession No. U89363.1 with the alpha-subunit encoded by GenBank Accession No. X64360.
Blast alignment of the beta-subunit encoded by GenBank Accession No. U89363.1 with the beta-subunit encoded by GenBank Accession No. X64360.
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