Spacers to increase the expression of recombinant fusion...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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Reexamination Certificate

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07943733

ABSTRACT:
The present invention relates to fusion proteins. The invention specifically relates to compositions and methods of Tf-based fusion proteins that demonstrate a high-level expression of transferrin (Tf)-based fusion proteins by inserting a helical linker between two protein domains.

REFERENCES:
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patent: 2009/0042777 (2009-02-01), Shen et al.
patent: 2005034877 (2005-04-01), None
Subramanian, et al., Albinterferon alpha-2b: a genetic fusion protein for the treatment of chronic hepatitis C. Nat. Biotechnol. 25: 1411-1419 (2007).
Pyror, et al., High-level expression of soluble protein inEscherichia coliusing a His6-Tag and maltos-bindingprotein double-affinity fusion system. Protein Expr. Purif. 10:309-319 (1997).
Maeda, et al., Engineering of functional chimeric protein Gvargula luciferase. Anal. Biochem. 249: 147-152 (1997).
Arai, et al., Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Eng. 14: 529-532 (2001).
Bai, et al., Improving the oral efficacy of recombinant granulocyte colony-stimulating factor and transferrin fusion protein by space optimization. Pharm. Res. 23: 2116-2121 (2006).
Jullien, et al., Regulation of Cre recombinase by ligand-induced complementation of inactive fragments. Nucleic Acids Res 31: e131 (2003).
Maeda, et al., A bifunctional poly(ethylene glycol) hybrid of lamininrelated peptides. Biochem Biophys Res Commun 248: 485-9 (1998).
Bai, et al., Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent. Proc. Natl. Acad. Sci.102: 7292-7296 (2005).
International search report for corresponding PCT application PCT/US08/87721 lists the references above.

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