Enzymatic detection techniques

Details Enzymatic detection techniques Enzymatic detection techniques

2011-03-15

2011-03-15

Ford, Allison M. (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

C435S018000, C435S023000, C435S024000, C435S287700

Reexamination Certificate

active

07906276

ABSTRACT:
A diagnostic test kit for detecting the presence or quantity of an enzyme or enzyme inhibitor is provided. The diagnostic kit utilizes reactive complexes to facilitate the detection of the enzyme or enzyme inhibitor. The reactive complexes include a substrate joined (e.g., covalently bonded, physically adsorbed, etc.) to a reporter and specific binding member. In one embodiment, for example, a peptide, protein, or glycoprotein substrate is joined to a reporter (e.g., dyed latex particle) and specific binding member (e.g., biotinylated compound). In this embodiment, the substrate provides a cleavage target for a proteolytic enzyme. Specifically, upon contacting the reactive complexes, the proteolytic enzyme cleaves the substrate and releases the reporter and/or specific binding member. The signal exhibited by the released reporters may then be used to indicate the presence or quantity of an enzyme or enzyme inhibitor within the test sample.

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