Method of classifying and counting leukocytes

Details Method of classifying and counting leukocytes Method of classifying and counting leukocytes

2011-04-12

2011-04-12

Gitomer, Ralph (Department: 1657)

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Concentration, separation, or purification of micro-organisms

C436S063000, C436S172000

Reexamination Certificate

active

07923229

ABSTRACT:
A method for classifying and counting leukocytes, which comprises steps of: staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent, with a fluorescent dye; introducing the sample containing the stained cells into a flow cytometer to measure first scattered light, second scattered light different from the first scattered light and fluorescence of the respective cells; obtaining scattered light peak intensities and scattered light widths of the respective cells based on the measured first scattered light, obtaining scattered light intensities of the respective cells based on the measured second scattered light, and obtaining fluorescence intensities of the respective cells based on the measured fluorescence light; classifying the cells into a first group and a second group based on the scattered light peak intensities and the scattered light widths, the first group including leukocytes and second group including platelet clumps; classifying the leukocytes included in the first group into at least lymphocytes, monocytes and granulocytes based on the scattered light intensities and the fluorescence intensities; and counting the classified lymphocytes, the classified monocytes and the classified granulocytes.

REFERENCES:
patent: 5891733 (1999-04-01), Inoue
patent: 6004816 (1999-12-01), Mizukami et al.
patent: 7625730 (2009-12-01), Tsuji et al.
patent: 2001/0049091 (2001-12-01), Thompson et al.
patent: 2003/0219850 (2003-11-01), Tsuji et al.
patent: 0 617 281 (1994-09-01), None
patent: 0 844 481 (1998-05-01), None
patent: 5-99919 (1993-04-01), None
patent: 6-273413 (1994-09-01), None
patent: 7-503792 (1995-04-01), None
patent: 10-206423 (1998-08-01), None
patent: 2002-148261 (2002-05-01), None
Diamond et al, “Flow Cytometry in the Diagnosis and Classification of Malignant Lymphoma and Leukemia”, Cancer, Sep. 15, 1982, vol. 50, No. 6, pp. 1122-1135.
Petriz et al, “Relevance of Forward Scatter and Side Scatter in Aneuploidy Detection by Flow Cytometry”, Analytical Cellular Pathology, vol. 10, No. 3, 1996, pp. 243-252.
Oelschlaegel et al, “Flow cytometric DNA-quantification of three-color immunophenotyped cells for subpopulation specific determination of aneuploidy and proliferation”, (J. of Immun. Methods), vol. 253, 2001, pp. 145-152.

Affiliated with

Also associated with

Rating

Method of classifying and counting leukocytes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of classifying and counting leukocytes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of classifying and counting leukocytes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2622194